Effect of Estramustine Phosphate on the Assembly of Isolated Bovine Brain Microtubules and Fast Axonal Transport in the Frog Sciatic Nerve

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Estramustine phosphate (0.01 to 0.5 nriM), an estradiol mustard derivative used in the therapy of prostatic carcinoma, inhibited the assembly of brain microtubule proteins in vitro and disassembled preformed microtubules. In the presence of estramustine phosphate, the minimum microtubule-protein concentration sufficient for the assembly of microtubules was increased. Low concentrations of taxoi (20 μM) completely reversed the inhibition of assembly by estramustine phosphate. The effects were specific to estramustine phosphate since neither estradiol 170-phosphate, the hormonal moiety of the drug, nor nornitrogen mustard, the alkylating moiety, had any effect on assembly. Estramustine phosphate (0.1 to 0.5 HIM) was also found to reversibly inhibit fast axonal transport in the frog sciatic nerve. The nerve content of adenosine triphosphate, adenosine diphosphate, and adenosine monophosphate was not significantly affected by estramustine phosphate. Our results suggest that the cytotoxic action of estramustine phosphate could be dependent partially on an interaction with microtubules, probably via the microtubule-associated proteins.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Göteborgs universitet
  • AB Leo
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cellbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)2234-2239
Antal sidor6
TidskriftCancer Research
Volym45
Utgivningsnummer5
StatusPublished - 1985 maj 1
PublikationskategoriForskning
Peer review utfördJa