Effect of ultraviolet-radiation and leakage of RB-86+ in guard-cells of Vicia-Faba
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The effects of UV-C (254 nm), UV-A (365 nm) and broad-band UV (280-380 nm) on guard cells of Vicia faba L. cv. Long Pod were investigated in the presence of white light (450 .mu.mol m-2 s-1). UV-C (7 .mu.mol m-2 s-1) was found to cause leakage of 86Rb+ from guard cells, while UV-A (0.3 .mu.mol m-2 s-1) stimulated increased uptake in these cells. A relatively small stimulatory effect was observed by broad-band UV (3 .mu.mol m-2 s-1) during the first 30 min of irradiation with an apparent equilibration of influx and efflux thereafter. Leakage of 86Rb+ from guard cells continued despite the removal of UV-C and an increase in the amount of white light from 450 to 1500 .mu.mol m-2 s-1, suggesting that membranes were irreversibly damaged. Irradiation of guard cells UV-C for 30, 45 and 90 min indicated that these cells began to be affected already by 30 min UV-C irradiation.