Effects of pentobarbital on GABA-activated currents in acutely-isolated rat dentate gyrus granule neurons
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Granule neurons from the rat dentate gyrus were acutely isolated and whole-cell currents recorded. Maximal enhancement of 7 muM gamma-aminobutyric acid (GABA; EC30) evoked currents was obtained with 100 muM pentobarbital where the peak-current was 2.1 +/- 0.2 of control. One hundred muM pentobarbital alone evoked no current response whereas I mM pentobarbital elicited a current response that was 0.4 +/- 0.2 of the 100 muM GABA-activated peak current. In 100 muM pentobarbital, the GABA EC50 value shifted from 14 to 3 muM but the peak-saturating-current value was not altered. An off-current was recorded on removal of 100 muM and higher pentobarbital concentrations. Ten mM pentobarbital abolished the peak-current response to 7 and 100 muM GABA. The results show that in the granule neurons the drug potency differs for the different effects of pentobarbital at GABA(A) receptors with the modulatory and inhibitory effects requiring lower concentrations than the direct activation of the receptors. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
|Enheter & grupper|
Ämnesklassifikation (UKÄ) – OBLIGATORISK
|Status||Published - 2003|
|Peer review utförd||Ja|