Effects of phosphorus in growth media on biomineralization and cell surface properties of marine cyanobacteria synechococcus

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Effects of phosphorus in growth media on biomineralization and cell surface properties of marine cyanobacteria synechococcus. / Paulo, Carlos; Kenney, Janice P.L.; Persson, Per; Dittrich, Maria.

I: Geosciences (Switzerland), Vol. 8, Nr. 12, 471, 01.12.2018.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T1 - Effects of phosphorus in growth media on biomineralization and cell surface properties of marine cyanobacteria synechococcus

AU - Paulo, Carlos

AU - Kenney, Janice P.L.

AU - Persson, Per

AU - Dittrich, Maria

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Through geological time, cyanobacterial picoplankton have impacted the global carbon cycle by sequestrating CO 2 and forming authigenic carbonate minerals. Various studies have emphasized the cyanobacterial cell envelopes as nucleation sites for calcium carbonate formation. Little is known, however, about how environmental conditions (e.g., nutrient content) trigger a cell surface and its properties and, consequently, influence biomineralization. Our study aims to understand how phosphorus (P) concentration impacts the properties of cell surfaces and cell–mineral interactions. Changes to the surface properties of marine Synechococcus strains grown under various P conditions were characterized by potentiometric titrations, X-ray photoelectron spectroscopy (XPS), and tip-enhanced Raman spectroscopy (TERS). Biomineralization experiments were performed using cyanobacterial cells, which were grown under different P concentrations and exposed to solutions slightly oversaturated with respect to calcium carbonate. We observed the changes induced by different P conditions in the macromolecular composition of the cyanobacteria cell envelope and its consequences for biomineralization. The modified properties of cell surfaces were linked to carbonate precipitation rates and mineral morphology from biomineralization experiments. Our analysis shows that the increase of phosphoryl groups and surface charge, as well as the relative proportion of polysaccharides and proteins, can impact carbonate precipitation by picocyanobacteria.

AB - Through geological time, cyanobacterial picoplankton have impacted the global carbon cycle by sequestrating CO 2 and forming authigenic carbonate minerals. Various studies have emphasized the cyanobacterial cell envelopes as nucleation sites for calcium carbonate formation. Little is known, however, about how environmental conditions (e.g., nutrient content) trigger a cell surface and its properties and, consequently, influence biomineralization. Our study aims to understand how phosphorus (P) concentration impacts the properties of cell surfaces and cell–mineral interactions. Changes to the surface properties of marine Synechococcus strains grown under various P conditions were characterized by potentiometric titrations, X-ray photoelectron spectroscopy (XPS), and tip-enhanced Raman spectroscopy (TERS). Biomineralization experiments were performed using cyanobacterial cells, which were grown under different P concentrations and exposed to solutions slightly oversaturated with respect to calcium carbonate. We observed the changes induced by different P conditions in the macromolecular composition of the cyanobacteria cell envelope and its consequences for biomineralization. The modified properties of cell surfaces were linked to carbonate precipitation rates and mineral morphology from biomineralization experiments. Our analysis shows that the increase of phosphoryl groups and surface charge, as well as the relative proportion of polysaccharides and proteins, can impact carbonate precipitation by picocyanobacteria.

KW - Calcium carbonate biomineralization

KW - Carbonate

KW - Cyanobacteria

KW - Infrared spectroscopy

KW - Phosphorus

KW - Synechococcus cells

KW - Tip-enhanced Raman spectroscopy

KW - X-ray photoelectron spectroscopy

U2 - 10.3390/geosciences8120471

DO - 10.3390/geosciences8120471

M3 - Article

AN - SCOPUS:85062385928

VL - 8

JO - Geosciences (Switzerland)

JF - Geosciences (Switzerland)

SN - 2076-3263

IS - 12

M1 - 471

ER -