Effects of Salts on Internal DNA Pressure and Mechanical Properties of Phage Capsids.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg(2+) and Sp(4+)). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage ϕ29 and herpes simplex virus type 1 particles.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysikalisk kemi
  • Biologiska vetenskaper
Originalspråkengelska
Sidor (från-till)18-23
TidskriftJournal of Molecular Biology
Volym405
StatusPublished - 2011
PublikationskategoriForskning
Peer review utfördJa