Electron microscopy imaging of proteins on gallium phosphide semiconductor nanowires.

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Electron microscopy imaging of proteins on gallium phosphide semiconductor nanowires. / Hjort, Martin; Bauer, Mikael; Gunnarsson, Stefan; Mårsell, Erik; Zakharov, Alexei; Karlsson, Gunnel; Sanfins, Elodie; Prinz, Christelle; Wallenberg, Reine; Cedervall, Tommy; Mikkelsen, Anders.

I: Nanoscale, Vol. 8, Nr. 7, 2016, s. 3936-3943.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Hjort M, Bauer M, Gunnarsson S, Mårsell E, Zakharov A, Karlsson G et al. Electron microscopy imaging of proteins on gallium phosphide semiconductor nanowires. Nanoscale. 2016;8(7):3936-3943. https://doi.org/10.1039/c5nr08888g

Author

Hjort, Martin ; Bauer, Mikael ; Gunnarsson, Stefan ; Mårsell, Erik ; Zakharov, Alexei ; Karlsson, Gunnel ; Sanfins, Elodie ; Prinz, Christelle ; Wallenberg, Reine ; Cedervall, Tommy ; Mikkelsen, Anders. / Electron microscopy imaging of proteins on gallium phosphide semiconductor nanowires. I: Nanoscale. 2016 ; Vol. 8, Nr. 7. s. 3936-3943.

RIS

TY - JOUR

T1 - Electron microscopy imaging of proteins on gallium phosphide semiconductor nanowires.

AU - Hjort, Martin

AU - Bauer, Mikael

AU - Gunnarsson, Stefan

AU - Mårsell, Erik

AU - Zakharov, Alexei

AU - Karlsson, Gunnel

AU - Sanfins, Elodie

AU - Prinz, Christelle

AU - Wallenberg, Reine

AU - Cedervall, Tommy

AU - Mikkelsen, Anders

PY - 2016

Y1 - 2016

N2 - We have imaged GaP nanowires (NWs) incubated with human laminin, serum albumin (HSA), and blood plasma using both cryo-transmission electron microscopy and synchrotron based X-ray photoemission electron microscopy. This extensive imaging methodology simultaneously reveals structural, chemical and morphological details of individual nanowires and the adsorbed proteins. We found that the proteins bind to NWs, forming coronas with thicknesses close to the proteins' hydrodynamic diameters. We could directly image how laminin is extending from the NWs, maximizing the number of proteins bound to the NWs. NWs incubated with both laminin and HSA show protein coronas with a similar appearance to NWs incubated with laminin alone, indicating that the presence of HSA does not affect the laminin conformation on the NWs. In blood plasma, an intermediate sized corona around the NWs indicates a corona with a mixture of plasma proteins. The ability to directly visualize proteins on nanostructures in situ holds great promise for assessing the conformation and thickness of the protein corona, which is key to understanding and predicting the properties of engineered nanomaterials in a biological environment.

AB - We have imaged GaP nanowires (NWs) incubated with human laminin, serum albumin (HSA), and blood plasma using both cryo-transmission electron microscopy and synchrotron based X-ray photoemission electron microscopy. This extensive imaging methodology simultaneously reveals structural, chemical and morphological details of individual nanowires and the adsorbed proteins. We found that the proteins bind to NWs, forming coronas with thicknesses close to the proteins' hydrodynamic diameters. We could directly image how laminin is extending from the NWs, maximizing the number of proteins bound to the NWs. NWs incubated with both laminin and HSA show protein coronas with a similar appearance to NWs incubated with laminin alone, indicating that the presence of HSA does not affect the laminin conformation on the NWs. In blood plasma, an intermediate sized corona around the NWs indicates a corona with a mixture of plasma proteins. The ability to directly visualize proteins on nanostructures in situ holds great promise for assessing the conformation and thickness of the protein corona, which is key to understanding and predicting the properties of engineered nanomaterials in a biological environment.

U2 - 10.1039/c5nr08888g

DO - 10.1039/c5nr08888g

M3 - Article

VL - 8

SP - 3936

EP - 3943

JO - Nanoscale

JF - Nanoscale

SN - 2040-3372

IS - 7

ER -