Electron microscopy of native cartilage oligomeric matrix protein purified from the Swarm rat chondrosarcoma reveals a five-armed structure

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Abstract

Cartilage oligomeric matrix protein was isolated in the native state from the Swarm rat chondrosarcoma. A crucial step was its selective extraction with EDTA-containing buffer. The purified protein was subjected to electron microscopy using rotary shadowing and negative stain. The images allowed the construction of a structural model. The bouquet-like protein consists of five 28-nm-long arms containing a peripheral globular domain, a flexible strand, and a central assembly domain, where the five arms meet in a cylindrical structure.

Detaljer

Författare
  • Matthias Mörgelin
  • Dick Heinegård
  • Jürgen Engel
  • Mats Paulsson
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Infektionsmedicin
  • Reumatologi och inflammation
Originalspråkengelska
Sidor (från-till)6137-6141
TidskriftJournal of Biological Chemistry
Volym267
Utgåva nummer9
StatusPublished - 1992
PublikationskategoriForskning
Peer review utfördJa