Emi2 Is Essential for Mouse Spermatogenesis

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The meiotic functions of Emi2, an inhibitor of the APC/C complex, have been best characterized in oocytes where it mediates metaphase II arrest as a component of the cytostatic factor. We generated knockout mice to determine the in vivo functions of Emi2—in particular, its functions in the testis, where Emi2 is expressed at high levels. Male and female Emi2 knockout mice are viable but sterile, indicating that Emi2 is essential for meiosis but dispensable for embryonic development and mitotic cell divisions. We found that, besides regulating cell-cycle arrest in mouse eggs, Emi2 is essential for meiosis I progression in spermatocytes. In the absence of Emi2, spermatocytes arrest in early diplotene of prophase I. This arrest is associated with decreased Cdk1 activity and was partially rescued by a knockin mouse model of elevated Cdk1 activity. Additionally, we detected expression of Emi2 in spermatids and sperm, suggesting potential post-meiotic functions for Emi2.

Detaljer

Författare
  • Lakshmi Gopinathan
  • Radoslaw Szmyd
  • Diana Low
  • M. Kasim Diril
  • Heng Yu Chang
  • Vincenzo Coppola
  • Kui Liu
  • Lino Tessarollo
  • Ernesto Guccione
  • Ans M.M. van Pelt
  • Philipp Kaldis
Externa organisationer
  • Institute of Molecular and Cell Biology
  • National University of Singapore
  • Dokuz Eylül University
  • Taipei Medical University
  • National Cancer Institute at Frederick
  • Ohio State University
  • Göteborgs universitet
  • Academic Medical Center
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)697-708
Antal sidor12
TidskriftCell Reports
Volym20
Utgivningsnummer3
StatusPublished - 2017 jul 18
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa