Endogenous beta-cell CART regulates insulin secretion and transcription of beta-cell genes

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Impaired beta-cell function is key to the development of type 2 diabetes. Cocaine- and amphetamine-regulated transcript (CART) is an islet peptide with insulinotropic and glucagonostatic properties. Here we studied the role of endogenous CART in beta-cell function. CART silencing in INS-1 (832/13) beta-cells reduced insulin secretion and production, ATP levels and beta-cell exocytosis. This was substantiated by reduced expression of several exocytosis genes, as well as reduced expression of genes important for insulin secretion and processing. In addition, CART silencing reduced the expression of a network of transcription factors essential for beta-cell function. Moreover, in RNAseq data from human islet donors, CARTPT expression levels correlated with insulin, exocytosis genes and key beta-cell transcription factors. Thus, endogenous beta-cell CART regulates insulin expression and secretion in INS-1 (832/13) cells, via actions on the exocytotic machinery and a network of beta-cell transcription factors. We conclude that CART is important for maintaining the beta-cell phenotype.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Skåne University Hospital
  • University of Geneva
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)52-60
TidskriftMolecular and Cellular Endocrinology
Volym447
Tidigt onlinedatum2017 feb 24
StatusPublished - 2017
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Liliya Shcherbina, 2017, Lund: Lund University, Faculty of Medicine. 107 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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