Engineered human mesenchymal stem cells for neuroblastoma therapeutics

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Drug-resistant neuroblastoma remains a major challenge in paediatric oncology and novel and less toxic therapeutic approaches are urgently needed to improve survival and reduce the side effects of traditional therapeutic interventions. Mesenchymal stem cells (MSCs) are an attractive candidate for cell and gene therapy since they are recruited by and able to infiltrate tumours. This feature has been exploited by creating genetically modified MSCs that are able to combat cancer by delivering therapeutic molecules. Whether neuroblastomas attract systemically delivered MSCs is still controversial. We investigated whether MSCs engineered to express tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) could: i) cause death of classic and primary neuroblastoma cell lines in vitro; ii) migrate to tumour sites in vivo; and iii) reduce neuroblastoma growth in xenotrans-plantation experiments. We observed that classic and primary neuroblastoma cell lines expressing death receptors could be killed by TRAIL-loaded MSCs in vitro. When injected in the peritoneum of neuroblastoma-bearing mice, TRAIL-MSCs migrated to tumour sites, but were unable to change the course of cancer development. These results indicated that MSCs have the potential to be used to deliver drugs in neuroblastoma patients, but more effective biopharmaceuticals should be used instead of TRAIL.

Detaljer

Författare
  • Valentina Nieddu
  • Roberta Piredda
  • Daniel Bexell
  • Jack Barton
  • John Anderson
  • Neil Sebire
  • Krishna Kolluri
  • Sam M. Janes
  • Emmanouil Karteris
  • Arturo Sala
Enheter & grupper
Externa organisationer
  • Brunel University London
  • University College London
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)35-42
Antal sidor8
TidskriftOncology Reports
Volym42
Utgåva nummer1
StatusPublished - 2019
PublikationskategoriForskning
Peer review utfördJa