Evaluation of the influence of CO2 on hydrogen production by Caldicellulosiruptor saccharolyticus

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Evaluation of the influence of CO2 on hydrogen production by Caldicellulosiruptor saccharolyticus. / Willquist, Karin; Claassen, Pieternel A. M.; van Niel, Ed.

I: International Journal of Hydrogen Energy, Vol. 34, Nr. 11, 2009, s. 4718-4726.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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TY - JOUR

T1 - Evaluation of the influence of CO2 on hydrogen production by Caldicellulosiruptor saccharolyticus

AU - Willquist, Karin

AU - Claassen, Pieternel A. M.

AU - van Niel, Ed

PY - 2009

Y1 - 2009

N2 - Stripping gas is generally used to improve hydrogen yields in fermentations. Since CO2 is relatively easy to separate from hydrogen it could be an interesting stripping gas. However, a higher partial CO2 pressure is accompanied with an increased CO2 uptake in the liquid, where it hydrolyses and induces an increased requirement of NaOH to maintain the pH. This enhances the osmotic pressure in the culture by 30%, which inhibited the growth of Caldicellulosiruptor saccharolyticus. indications for this conclusion are: i) inhibition could almost completely be circumvented by reducing the bicarbonate through decreasing the pH (from 6.5 to 5.5), ii) Growth rates were reduced by 60 +/- 10% at an osmotic pressure of 0.218 +/- 0.005 osm/kg H2O independently of the stripping gas, iii) Increased extracellular DNA and protein concentrations were observed as a function of the osmotic pressure. In addition to growth inhibition, the increased sodium bicarbonate in the effluent will contribute to a negative environmental impact when applied at industrial scale. (C) 2009 International Association for Hydrogen Energy. Published by Elsevier Ltd. All lights reserved.

AB - Stripping gas is generally used to improve hydrogen yields in fermentations. Since CO2 is relatively easy to separate from hydrogen it could be an interesting stripping gas. However, a higher partial CO2 pressure is accompanied with an increased CO2 uptake in the liquid, where it hydrolyses and induces an increased requirement of NaOH to maintain the pH. This enhances the osmotic pressure in the culture by 30%, which inhibited the growth of Caldicellulosiruptor saccharolyticus. indications for this conclusion are: i) inhibition could almost completely be circumvented by reducing the bicarbonate through decreasing the pH (from 6.5 to 5.5), ii) Growth rates were reduced by 60 +/- 10% at an osmotic pressure of 0.218 +/- 0.005 osm/kg H2O independently of the stripping gas, iii) Increased extracellular DNA and protein concentrations were observed as a function of the osmotic pressure. In addition to growth inhibition, the increased sodium bicarbonate in the effluent will contribute to a negative environmental impact when applied at industrial scale. (C) 2009 International Association for Hydrogen Energy. Published by Elsevier Ltd. All lights reserved.

KW - Biohydrogen production

KW - Osmotic pressure

KW - CO2

KW - Stripping gas

KW - Caldicellulosiruptor saccharolyticus

U2 - 10.1016/j.ijhydene.2009.03.056

DO - 10.1016/j.ijhydene.2009.03.056

M3 - Article

VL - 34

SP - 4718

EP - 4726

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

SN - 1879-3487

IS - 11

ER -