Evidence for a morphologically distinct and functionally robust cell type in the proximal tubules of human kidney.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Acute tubular necrosis (ATN), elicited by ischemia and/or toxicity, is a potentially life-threatening condition. Histologically, ATN corresponds to necrosis and detachment of renal tubular epithelial cells. However, the tubules possess a considerable regenerative capacity and may be restored. We have previously identified a scattered population of progenitor-like cells within the proximal tubules, sharing marker expression with the parietal epithelial cells of Bowman's capsule as well as with renal tubules regenerating after ATN. In the present analysis, we use transmission electron microscopy, immunoelectron microscopy and immunofluorescence of human kidney cortex to further explore these cells. We demonstrate that the cells are smaller and have drastically fewer mitochondria than the surrounding proximal tubule cells. They also display strong expression of several structural proteins such as vimentin, collagen-7A1 and the tight junction protein claudin-1. To functionally assess these cells, we also developed a novel human kidney explant model of ATN demonstrating that the cells are more resilient to injury than the surrounding proximal tubular cells. Taken together the results suggest a novel robust cell type with a contrasting biological role to that of the bulk of proximal tubular epithelium.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cancer och onkologi
Originalspråkengelska
Sidor (från-till)382-393
TidskriftHuman Pathology
Volym45
Utgivningsnummer2
StatusPublished - 2014
PublikationskategoriForskning
Peer review utfördJa

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Relaterad forskningsoutput

Jennifer Hansson, 2016, Department of Laboratory Medicine, Lund University. 88 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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