Evidence for substrate binding of a recombinant thermostable xylanase originating from Rhodothermus marinus

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Evidence for substrate binding of a recombinant thermostable xylanase originating from Rhodothermus marinus. / Nordberg Karlsson, Eva; Bartonek-Roxå, Eva; Holst, Olle.

I: FEMS Microbiology Letters, Vol. 168, Nr. 1, 01.11.1998, s. 1-7.

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T1 - Evidence for substrate binding of a recombinant thermostable xylanase originating from Rhodothermus marinus

AU - Nordberg Karlsson, Eva

AU - Bartonek-Roxå, Eva

AU - Holst, Olle

PY - 1998/11/1

Y1 - 1998/11/1

N2 - The xyn1 encoded 5 domain xylanase from the thermophilic bacterium Rhodothermus marinus binds specifically to xylan, β-glucan and amorphous but not crystalline cellulose. Our results show that the binding is mediated by the full length xylanase, but not by the catalytic domain only. Based on similarities concerning both predicted secondary structure and binding specificity found with one cellulose binding domain of CenC from Cellulomonas fimi, we suggest that the binding is mediated by the two N-terminally repeated domains. Copyright (C) 1998 Federation of European Microbiological Societies.

AB - The xyn1 encoded 5 domain xylanase from the thermophilic bacterium Rhodothermus marinus binds specifically to xylan, β-glucan and amorphous but not crystalline cellulose. Our results show that the binding is mediated by the full length xylanase, but not by the catalytic domain only. Based on similarities concerning both predicted secondary structure and binding specificity found with one cellulose binding domain of CenC from Cellulomonas fimi, we suggest that the binding is mediated by the two N-terminally repeated domains. Copyright (C) 1998 Federation of European Microbiological Societies.

KW - Carbohydrate binding domain

KW - Rhodothermus marinus

KW - Thermostable

KW - Xylanase

UR - http://www.scopus.com/inward/record.url?scp=0032211894&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(98)00404-2

DO - 10.1016/S0378-1097(98)00404-2

M3 - Article

C2 - 9812357

AN - SCOPUS:0032211894

VL - 168

SP - 1

EP - 7

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 1574-6968

IS - 1

ER -