Exposure to Perfluoroalkyl Substances and Sperm DNA Global Methylation in Arctic and European Populations

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Exposure to Perfluoroalkyl Substances and Sperm DNA Global Methylation in Arctic and European Populations. / Leter, Giorgio; Consales, Claudia; Eleuteri, Patrizia; Uccelli, Raffaella; Specht, Ina O.; Toft, Gunnar; Moccia, Tania; Budillon, Alfredo; Jönsson, Bo A; Lindh, Christian; Giwercman, Aleksander; Pedersen, Henning S.; Ludwicki, Jan K.; Zviezdai, Valentyna; Heederik, Dick; Bonde, Jens Peter E.; Spano, Marcello.

I: Environmental and Molecular Mutagenesis, Vol. 55, Nr. 7, 2014, s. 591-600.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Leter, G, Consales, C, Eleuteri, P, Uccelli, R, Specht, IO, Toft, G, Moccia, T, Budillon, A, Jönsson, BA, Lindh, C, Giwercman, A, Pedersen, HS, Ludwicki, JK, Zviezdai, V, Heederik, D, Bonde, JPE & Spano, M 2014, 'Exposure to Perfluoroalkyl Substances and Sperm DNA Global Methylation in Arctic and European Populations', Environmental and Molecular Mutagenesis, vol. 55, nr. 7, s. 591-600. https://doi.org/10.1002/em.21874

APA

Leter, G., Consales, C., Eleuteri, P., Uccelli, R., Specht, I. O., Toft, G., ... Spano, M. (2014). Exposure to Perfluoroalkyl Substances and Sperm DNA Global Methylation in Arctic and European Populations. Environmental and Molecular Mutagenesis, 55(7), 591-600. https://doi.org/10.1002/em.21874

CBE

Leter G, Consales C, Eleuteri P, Uccelli R, Specht IO, Toft G, Moccia T, Budillon A, Jönsson BA, Lindh C, Giwercman A, Pedersen HS, Ludwicki JK, Zviezdai V, Heederik D, Bonde JPE, Spano M. 2014. Exposure to Perfluoroalkyl Substances and Sperm DNA Global Methylation in Arctic and European Populations. Environmental and Molecular Mutagenesis. 55(7):591-600. https://doi.org/10.1002/em.21874

MLA

Vancouver

Author

Leter, Giorgio ; Consales, Claudia ; Eleuteri, Patrizia ; Uccelli, Raffaella ; Specht, Ina O. ; Toft, Gunnar ; Moccia, Tania ; Budillon, Alfredo ; Jönsson, Bo A ; Lindh, Christian ; Giwercman, Aleksander ; Pedersen, Henning S. ; Ludwicki, Jan K. ; Zviezdai, Valentyna ; Heederik, Dick ; Bonde, Jens Peter E. ; Spano, Marcello. / Exposure to Perfluoroalkyl Substances and Sperm DNA Global Methylation in Arctic and European Populations. I: Environmental and Molecular Mutagenesis. 2014 ; Vol. 55, Nr. 7. s. 591-600.

RIS

TY - JOUR

T1 - Exposure to Perfluoroalkyl Substances and Sperm DNA Global Methylation in Arctic and European Populations

AU - Leter, Giorgio

AU - Consales, Claudia

AU - Eleuteri, Patrizia

AU - Uccelli, Raffaella

AU - Specht, Ina O.

AU - Toft, Gunnar

AU - Moccia, Tania

AU - Budillon, Alfredo

AU - Jönsson, Bo A

AU - Lindh, Christian

AU - Giwercman, Aleksander

AU - Pedersen, Henning S.

AU - Ludwicki, Jan K.

AU - Zviezdai, Valentyna

AU - Heederik, Dick

AU - Bonde, Jens Peter E.

AU - Spano, Marcello

PY - 2014

Y1 - 2014

N2 - Perfluoroalkyl substances (PFASs) are widely used in a variety of industrial processes and products, and have been detected globally in humans and wildlife. PFASs are suspected to interfere with endocrine signaling and to adversely affect human reproductive health. The aim of the present study was to investigate the associations between exposure to PFASs and sperm global methylation levels in a population of non-occupationally exposed fertile men. Measurements of PFASs in serum from 262 partners of pregnant women from Greenland, Poland and Ukraine, were also carried out by liquid chromatography tandem mass spectrometry. Perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorohexane sulfonic acid (PFHxS), and perfluorononanoic acid (PFNA) were detected in 97% of the blood samples. Two surrogate markers were used to assess DNA global methylation levels in semen samples from the same men: (a) average DNA methylation level in repetitive DNA sequences (Alu, LINE-1, Sat alpha) quantified by PCR-pyrosequencing after bisulfite conversion; (b) flow cytometric immunodetection of 5-methyl-cytosines. After multivariate linear regression analysis, no major consistent associations between PFASs exposure and sperm DNA global methylation endpoints could be detected. However, since weak but statistically significant associations of different PFASs with DNA hypo- and hypermethylation were found in some of the studied populations, effects of PFASs on sperm epigenetic processes cannot be completely excluded, and this issue warrants further investigation. (C) 2014 Wiley Periodicals, Inc.

AB - Perfluoroalkyl substances (PFASs) are widely used in a variety of industrial processes and products, and have been detected globally in humans and wildlife. PFASs are suspected to interfere with endocrine signaling and to adversely affect human reproductive health. The aim of the present study was to investigate the associations between exposure to PFASs and sperm global methylation levels in a population of non-occupationally exposed fertile men. Measurements of PFASs in serum from 262 partners of pregnant women from Greenland, Poland and Ukraine, were also carried out by liquid chromatography tandem mass spectrometry. Perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorohexane sulfonic acid (PFHxS), and perfluorononanoic acid (PFNA) were detected in 97% of the blood samples. Two surrogate markers were used to assess DNA global methylation levels in semen samples from the same men: (a) average DNA methylation level in repetitive DNA sequences (Alu, LINE-1, Sat alpha) quantified by PCR-pyrosequencing after bisulfite conversion; (b) flow cytometric immunodetection of 5-methyl-cytosines. After multivariate linear regression analysis, no major consistent associations between PFASs exposure and sperm DNA global methylation endpoints could be detected. However, since weak but statistically significant associations of different PFASs with DNA hypo- and hypermethylation were found in some of the studied populations, effects of PFASs on sperm epigenetic processes cannot be completely excluded, and this issue warrants further investigation. (C) 2014 Wiley Periodicals, Inc.

KW - perfluoroalkyl substances

KW - human spermatozoa

KW - DNA methylation

KW - pyrosequencing

KW - flow cytometry

KW - epidemiology

U2 - 10.1002/em.21874

DO - 10.1002/em.21874

M3 - Article

VL - 55

SP - 591

EP - 600

JO - Environmental and Molecular Mutagenesis

T2 - Environmental and Molecular Mutagenesis

JF - Environmental and Molecular Mutagenesis

SN - 1098-2280

IS - 7

ER -