Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein

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Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D2O for H2O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein gamma B-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature T-c can be described by the extended law of corresponding states for all H2O/D2O ratios, where scaling of the temperature by T-c or the reduced second virial coefficient accurately reproduces the binodal, spinodal, and osmotic compressibility. These findings highlight the impact of H2O/D2O substitution on gamma B-crystallin properties and warrant further investigations into the universality of this phenomenon and its underlying mechanisms.


  • Najet Mahmoudi
  • Lucia Casal-Dujat
  • Marie Jehannin
  • Corinne Jud
  • Anna Stradner
Enheter & grupper
Externa organisationer
  • University of Fribourg

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysikalisk kemi
  • Teoretisk kemi
Sidor (från-till)1610-1615
Antal sidor6
TidskriftThe Journal of Physical Chemistry Letters
Utgåva nummer9
StatusPublished - 2016 maj 5
Peer review utfördJa