Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant for human tissue, but suitable bioinks for producing translationally relevant tissue with complex geometries remain unidentified. Here, a tissue-specific hybrid bioink is described, composed of a natural polymer, alginate, reinforced with extracellular matrix derived from decellularized tissue (rECM). rECM has rheological and gelation properties beneficial for 3D bioprinting while retaining biologically inductive properties supporting tissue maturation ex vivo and in vivo. These bioinks are shear thinning, resist cell sedimentation, improve viability of multiple cell types, and enhance mechanical stability in hydrogels derived from them. 3D printed constructs generated from rECM bioinks suppress the foreign body response, are pro-angiogenic and support recipient-derived de novo blood vessel formation across the entire graft thickness in a murine model of transplant immunosuppression. Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. rECM bioinks are a promising new approach for generating functional human tissue using 3D bioprinting.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Ludwig-Maximilian University of Munich
  • University of Twente
  • Skåne University Hospital
  • Helmholtz Zentrum München
  • University Hospital Munich
  • University of Glasgow
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biomaterialvetenskap
  • Medicinteknik
Originalspråkengelska
Artikelnummere2005476
TidskriftAdvanced Materials
StatusE-pub ahead of print - 2020 dec 9
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Martina M. De Santis, 2021, Lund: Lund University, Faculty of Medicine. 95 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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