Extrachromosomal RNA-DNA complex containing long telomeric repeats in chironomids.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We have analysed an extracted RNase sensitive fraction containing telomeric repeat sequences in the telomerase negative dipteran Chironomus tentans. It shows a slow and well-defined electophoretic migration corresponding to > 20 kb and is sensitive not only to RNase, but also to DNase. It hybridizes to both strands of the telomeric repeat with about equal intensities. DNA is probably the dominant component since the fraction is only slightly heavier than genomic DNA in isopycnic gradients but considerably lighter than RNA. It can, nevertheless, be shown to incorporate tritiated uridine. The material might represent another example of extrachromosomal telomeric repeats in telomerase negative cells.

Detaljer

Författare
  • Monika Rosén
  • I Kamnert
  • J-E Edström
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Genetik

Nyckelord

Originalspråkengelska
Sidor (från-till)167-174
TidskriftInsect Molecular Biology
Volym11
Utgåva nummer2
StatusPublished - 2002
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Rosén, M., 2001, Department of Genetics, Lund University. 130 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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