Fecal water from ileostomic patients consuming oat beta-glucan enhances immune responses in enterocytes

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Fecal water from ileostomic patients consuming oat beta-glucan enhances immune responses in enterocytes. / Ramakers, Julian D.; Volman, Julia J.; Biörklund, Maria; Onning, Gunilla; Mensink, Ronald P.; Plat, Jogchum.

I: Molecular Nutrition and Food Research, Vol. 51, Nr. 2, 2007, s. 211-220.

Forskningsoutput: TidskriftsbidragÖversiktsartikel

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Ramakers, Julian D. ; Volman, Julia J. ; Biörklund, Maria ; Onning, Gunilla ; Mensink, Ronald P. ; Plat, Jogchum. / Fecal water from ileostomic patients consuming oat beta-glucan enhances immune responses in enterocytes. I: Molecular Nutrition and Food Research. 2007 ; Vol. 51, Nr. 2. s. 211-220.

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TY - JOUR

T1 - Fecal water from ileostomic patients consuming oat beta-glucan enhances immune responses in enterocytes

AU - Ramakers, Julian D.

AU - Volman, Julia J.

AU - Biörklund, Maria

AU - Onning, Gunilla

AU - Mensink, Ronald P.

AU - Plat, Jogchum

PY - 2007

Y1 - 2007

N2 - Yeast, fungal, and dietary beta-glucans have immune-modulating effects in vitro and in vivo, as thought, mainly by affecting leukocytes; however, effects of oat beta-glucan on enterocytes have never been studied. As recognized, supplying oat beta-glucans as such to cells in culture directly is difficult because of solubility problems. Therefore, six ileostomic patients consumed, in random order, a control diet or an oat beta-glucan enriched diet (5 g) and from the collected ileostomic content, fecal water was prepared and added to two small intestinal cell lines (INT407, Caco-2) and two colon cell lines (HT29, T84) together with a cytokine cocktail (IL-IP + 1NF gamma + TNF alpha). Several parameters reflecting immune-modulation were measured. As compared to placebo fecal water, P-glucan enriched fecal water significantly increased IL-8 production in HT29 (5.0%; p = 0.046) and INT407 cells (22.0%; p=0.028). Intercellular adhesion molecule (ICAM)-1 expression increased in T84 (11.0%; p = 0.028) and Caco-2 cells (20.4%; p = 0.075). These immune-stimulating effects were confirmed by enhancement of inflammatory expression profiles, as determined with an antibody array. Our findings show immune enhancement by fecal water from ileostomic patients consuming oat P-glucan both in small intestinal and colon cell lines after stimulation, which is in agreement with documented effects in leukocytes. Whether these immune-stimulating effects on enterocytes contribute to the enhanced protection of the host against invading pathogens as observed both in animals and in humans, as well as the underlying mechanism, needs further evaluation.

AB - Yeast, fungal, and dietary beta-glucans have immune-modulating effects in vitro and in vivo, as thought, mainly by affecting leukocytes; however, effects of oat beta-glucan on enterocytes have never been studied. As recognized, supplying oat beta-glucans as such to cells in culture directly is difficult because of solubility problems. Therefore, six ileostomic patients consumed, in random order, a control diet or an oat beta-glucan enriched diet (5 g) and from the collected ileostomic content, fecal water was prepared and added to two small intestinal cell lines (INT407, Caco-2) and two colon cell lines (HT29, T84) together with a cytokine cocktail (IL-IP + 1NF gamma + TNF alpha). Several parameters reflecting immune-modulation were measured. As compared to placebo fecal water, P-glucan enriched fecal water significantly increased IL-8 production in HT29 (5.0%; p = 0.046) and INT407 cells (22.0%; p=0.028). Intercellular adhesion molecule (ICAM)-1 expression increased in T84 (11.0%; p = 0.028) and Caco-2 cells (20.4%; p = 0.075). These immune-stimulating effects were confirmed by enhancement of inflammatory expression profiles, as determined with an antibody array. Our findings show immune enhancement by fecal water from ileostomic patients consuming oat P-glucan both in small intestinal and colon cell lines after stimulation, which is in agreement with documented effects in leukocytes. Whether these immune-stimulating effects on enterocytes contribute to the enhanced protection of the host against invading pathogens as observed both in animals and in humans, as well as the underlying mechanism, needs further evaluation.

KW - beta-glucan

KW - oat

KW - intestinal epithelial cells

KW - interleukin-8

KW - ICAM-1

KW - ileostomy

U2 - 10.1002/mnfr.200600149

DO - 10.1002/mnfr.200600149

M3 - Review article

VL - 51

SP - 211

EP - 220

JO - Molecular Nutrition and Food Research

T2 - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4133

IS - 2

ER -