Filter-exchange PGSE NMR determination of cell membrane permeability.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.

Detaljer

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Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysikalisk kemi
Originalspråkengelska
Sidor (från-till)291-295
TidskriftJournal of Magnetic Resonance
Volym200
StatusPublished - 2009
PublikationskategoriForskning
Peer review utfördJa