Fimbrial lectins influence the chemokine repertoire in the urinary tract mucosa.

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Fimbrial lectins influence the chemokine repertoire in the urinary tract mucosa. / Godaly, Gabriela; Otto, Gisela; Burdick, M D; Strieter, R M; Svanborg, Catharina.

I: Kidney International, Vol. 71, Nr. 8, 2007, s. 778-786.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T1 - Fimbrial lectins influence the chemokine repertoire in the urinary tract mucosa.

AU - Godaly, Gabriela

AU - Otto, Gisela

AU - Burdick, M D

AU - Strieter, R M

AU - Svanborg, Catharina

PY - 2007

Y1 - 2007

N2 - The defense against mucosal infections relies on chemokines that recruit inflammatory cells to the mucosa. This study examined if the chemokine response to uro-pathogenic Escherichia coli is influenced by fimbrial expression. The CXC (CXCL1, CXCL5, CXCL8, CXCL9, CXCL10) and CC chemokines (CCL2, CCL3, CCL5) were quantified after in vitro infection of uro-epithelial cells with a fimbriated E. coli pyelonephritis isolate, or with P or type 1 fimbriated transformants of an avirulent E. coli K-12 strain. The response profile was shown to vary with the fimbrial type. Type 1 fimbriated E. coli elicited mainly CXCL1 and CXCL8, whereas P fimbriated E. coli stimulated CCL2 and CCL5 and class II were more potent chemokine inducers than class III P fimbriae. Chemokines were also quantified in urine samples from 73 patients with febrile urinary tract infection, and analyzed as a function of disease severity and fimbrial expression by the strain infecting each patient. A complex CXC and CC chemokine response was detected in patient urine, with a significant influence of the fimbrial type. The results show that virulence factors like fimbriae may modify the mucosal chemokine response. This mechanism may allow the host to adjust the inflammatory cell infiltrate to fit the infecting strain.

AB - The defense against mucosal infections relies on chemokines that recruit inflammatory cells to the mucosa. This study examined if the chemokine response to uro-pathogenic Escherichia coli is influenced by fimbrial expression. The CXC (CXCL1, CXCL5, CXCL8, CXCL9, CXCL10) and CC chemokines (CCL2, CCL3, CCL5) were quantified after in vitro infection of uro-epithelial cells with a fimbriated E. coli pyelonephritis isolate, or with P or type 1 fimbriated transformants of an avirulent E. coli K-12 strain. The response profile was shown to vary with the fimbrial type. Type 1 fimbriated E. coli elicited mainly CXCL1 and CXCL8, whereas P fimbriated E. coli stimulated CCL2 and CCL5 and class II were more potent chemokine inducers than class III P fimbriae. Chemokines were also quantified in urine samples from 73 patients with febrile urinary tract infection, and analyzed as a function of disease severity and fimbrial expression by the strain infecting each patient. A complex CXC and CC chemokine response was detected in patient urine, with a significant influence of the fimbrial type. The results show that virulence factors like fimbriae may modify the mucosal chemokine response. This mechanism may allow the host to adjust the inflammatory cell infiltrate to fit the infecting strain.

KW - urinary tract infection

KW - fimbriae

KW - chemokines

KW - Escherichia coli

U2 - 10.1038/sj.ki.5002076

DO - 10.1038/sj.ki.5002076

M3 - Article

VL - 71

SP - 778

EP - 786

JO - Kidney International

T2 - Kidney International

JF - Kidney International

SN - 1523-1755

IS - 8

ER -