Flow-through picoliter dispenser: A new approach for solvent elimination in FT-IR spectroscopy

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Flow-through picoliter dispenser: A new approach for solvent elimination in FT-IR spectroscopy. / Haberkorn, M; Frank, J; Harasek, M; Nilsson, Johan; Laurell, Thomas; Lendl, B.

I: Applied Spectroscopy, Vol. 56, Nr. 7, 2002, s. 902-908.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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TY - JOUR

T1 - Flow-through picoliter dispenser: A new approach for solvent elimination in FT-IR spectroscopy

AU - Haberkorn, M

AU - Frank, J

AU - Harasek, M

AU - Nilsson, Johan

AU - Laurell, Thomas

AU - Lendl, B

PY - 2002

Y1 - 2002

N2 - A new interface for FT-IR analysis of liquid samples on the basis of solvent elimination is presented. The approach is based on a piezoactuated flow-through microdispenser, a device built of two microstructured silicon wafers designed for micro-liquid handling. It could be verified during preliminary studies using a sequential injection (SI) system for automated liquid handling that the flow-through microdispenser as a possible interface for flow system-FT-IR analysis has the capability of meeting the demands of hyphenated miniaturized liquid handling systems (e.g., mu-HPLC, microhigh performance liquid chromatography), as it successfully provides highly stable, reliable and reproducible operating conditions for liquid handling in the picoliter range. Moreover, an increase in sensitivity for FT-IR measurements could be achieved, lowering the mass detection limit of sugars (such as the investigated sucrose) to 53 picograms. As is demonstrated on the example of an HPLC separation of a mixture of glucose and fructose, interfacing LC systems to FT-IR using a piezoactuated flow-through microdispenser is a feasible and promising approach.

AB - A new interface for FT-IR analysis of liquid samples on the basis of solvent elimination is presented. The approach is based on a piezoactuated flow-through microdispenser, a device built of two microstructured silicon wafers designed for micro-liquid handling. It could be verified during preliminary studies using a sequential injection (SI) system for automated liquid handling that the flow-through microdispenser as a possible interface for flow system-FT-IR analysis has the capability of meeting the demands of hyphenated miniaturized liquid handling systems (e.g., mu-HPLC, microhigh performance liquid chromatography), as it successfully provides highly stable, reliable and reproducible operating conditions for liquid handling in the picoliter range. Moreover, an increase in sensitivity for FT-IR measurements could be achieved, lowering the mass detection limit of sugars (such as the investigated sucrose) to 53 picograms. As is demonstrated on the example of an HPLC separation of a mixture of glucose and fructose, interfacing LC systems to FT-IR using a piezoactuated flow-through microdispenser is a feasible and promising approach.

KW - micro-liquid handling

KW - FT-IR

KW - infrared spectroscopy

KW - Fourier transform

KW - flow-through microdispenser

KW - solvent elimination

U2 - 10.1366/000370202760171581

DO - 10.1366/000370202760171581

M3 - Article

VL - 56

SP - 902

EP - 908

JO - Applied Spectroscopy

T2 - Applied Spectroscopy

JF - Applied Spectroscopy

SN - 1943-3530

IS - 7

ER -