Focal traumatic brain injury induces neuroplastic molecular responses in lumbar spinal cord

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Focal traumatic brain injury induces neuroplastic molecular responses in lumbar spinal cord. / Kononenko, Olga; Watanabe, Hiroyuki; Stålhandske, Lada; Zarelius, Ann; Clausen, Fredrik; Yakovleva, Tatiana; Bakalkin, Georgy; Marklund, Niklas.

I: Restorative Neurology and Neuroscience, Vol. 37, Nr. 2, 2019, s. 87-96.

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Kononenko, Olga ; Watanabe, Hiroyuki ; Stålhandske, Lada ; Zarelius, Ann ; Clausen, Fredrik ; Yakovleva, Tatiana ; Bakalkin, Georgy ; Marklund, Niklas. / Focal traumatic brain injury induces neuroplastic molecular responses in lumbar spinal cord. I: Restorative Neurology and Neuroscience. 2019 ; Vol. 37, Nr. 2. s. 87-96.

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TY - JOUR

T1 - Focal traumatic brain injury induces neuroplastic molecular responses in lumbar spinal cord

AU - Kononenko, Olga

AU - Watanabe, Hiroyuki

AU - Stålhandske, Lada

AU - Zarelius, Ann

AU - Clausen, Fredrik

AU - Yakovleva, Tatiana

AU - Bakalkin, Georgy

AU - Marklund, Niklas

PY - 2019

Y1 - 2019

N2 - Background/Objectives: Motor impairment induced by traumatic brain injury (TBI) may be mediated through changes in spinal molecular systems regulating neuronal plasticity. We assessed whether a focal controlled cortical impact (CCI) TBI in the rat alters expression of the Tgfb1, c-Fos, Bdnf, and Gap43 neuroplasticity genes in lumbar spinal cord. Approach/Methods: Adult male Sprague-Dawley rats (n = 8) were subjected to a right-side CCI over the anterior sensorimotor hindlimb representation area or sham-injury (n = 8). Absolute expression levels of Tgfb1, c-Fos, Bdnf, and Gapd43 genes were measured by droplet digital PCR in ipsi-and contralesional, dorsal and ventral quadrants of the L4 and L5 spinal cord. The neuronal activity marker c-Fos was analysed by immunohistochemistry in the dorsal L4 and L5 segments. The contra- vs. ipsilesional expression pattern was examined as the asymmetry index, AI. Results: The Tgfb1 mRNA levels were significantly higher in the CCI vs. sham-injured rats, and in the contra- vs. ipsilesional dorsal domains in the CCI group. The number of c-Fos-positive cells was elevated in the L4 and L5 segments; and on the contralesional compared to the ipsilesional side in the CCI group. The c-Fos AI in the dorsal laminae was significantly increased by CCI. Conclusions: The results support the hypothesis that focal TBI induces plastic alterations in the lumbar spinal cord that may contribute to either motor recovery or maladaptive motor responses.

AB - Background/Objectives: Motor impairment induced by traumatic brain injury (TBI) may be mediated through changes in spinal molecular systems regulating neuronal plasticity. We assessed whether a focal controlled cortical impact (CCI) TBI in the rat alters expression of the Tgfb1, c-Fos, Bdnf, and Gap43 neuroplasticity genes in lumbar spinal cord. Approach/Methods: Adult male Sprague-Dawley rats (n = 8) were subjected to a right-side CCI over the anterior sensorimotor hindlimb representation area or sham-injury (n = 8). Absolute expression levels of Tgfb1, c-Fos, Bdnf, and Gapd43 genes were measured by droplet digital PCR in ipsi-and contralesional, dorsal and ventral quadrants of the L4 and L5 spinal cord. The neuronal activity marker c-Fos was analysed by immunohistochemistry in the dorsal L4 and L5 segments. The contra- vs. ipsilesional expression pattern was examined as the asymmetry index, AI. Results: The Tgfb1 mRNA levels were significantly higher in the CCI vs. sham-injured rats, and in the contra- vs. ipsilesional dorsal domains in the CCI group. The number of c-Fos-positive cells was elevated in the L4 and L5 segments; and on the contralesional compared to the ipsilesional side in the CCI group. The c-Fos AI in the dorsal laminae was significantly increased by CCI. Conclusions: The results support the hypothesis that focal TBI induces plastic alterations in the lumbar spinal cord that may contribute to either motor recovery or maladaptive motor responses.

KW - c-Fos

KW - plasticity

KW - spinal cord

KW - Tgfb1

KW - Traumatic brain injury

U2 - 10.3233/RNN-180882

DO - 10.3233/RNN-180882

M3 - Article

VL - 37

SP - 87

EP - 96

JO - Restorative Neurology and Neuroscience

T2 - Restorative Neurology and Neuroscience

JF - Restorative Neurology and Neuroscience

SN - 1878-3627

IS - 2

ER -