Frequency and patterns of reassortment in natural influenza A virus infection in a reservoir host

TY - JOUR

T1 - Frequency and patterns of reassortment in natural influenza A virus infection in a reservoir host

AU - Wille, Michelle

AU - Tolf, Conny

AU - Avril, Alexis

AU - Latorre-Margalef, Neus

AU - Wallerström, Sofie

AU - Olsen, Björn

AU - Waldenström, Jonas

PY - 2013/8/15

Y1 - 2013/8/15

N2 - Influenza A viruses (IAV) can dramatically alter both genotype and phenotype at a rapid rate as a product of co-infection and reassortment. Avian IAV exhibit high levels of phylogenetic incongruence, suggesting high levels of reassortment in the virus reservoir. Using a natural-experimental system, we reconstructed relationships amongst 92 viruses across 15 subtypes from 10 Mallards in an autumn season. Phylogenetic analyses estimated that 56% of the isolated viruses were reassorted. Network analysis demonstrated different patterns of reassortment and limited exchange of segments between primary and secondary infections. No clear patterns of linkage between segments were found, and patterns within a season were likely the consequence of continued introduction of new constellations, high viral load and diversity in the wild bird reservoir, and co-infections. This is the first IAV study to implement multiple tools available for elucidating factors governing reassortment patterns in naturally infected Mallards.

AB - Influenza A viruses (IAV) can dramatically alter both genotype and phenotype at a rapid rate as a product of co-infection and reassortment. Avian IAV exhibit high levels of phylogenetic incongruence, suggesting high levels of reassortment in the virus reservoir. Using a natural-experimental system, we reconstructed relationships amongst 92 viruses across 15 subtypes from 10 Mallards in an autumn season. Phylogenetic analyses estimated that 56% of the isolated viruses were reassorted. Network analysis demonstrated different patterns of reassortment and limited exchange of segments between primary and secondary infections. No clear patterns of linkage between segments were found, and patterns within a season were likely the consequence of continued introduction of new constellations, high viral load and diversity in the wild bird reservoir, and co-infections. This is the first IAV study to implement multiple tools available for elucidating factors governing reassortment patterns in naturally infected Mallards.

KW - Anas platyrhynchos

KW - Evolution

KW - Influenza A virus

KW - Mallard

KW - Reassortment

KW - Sentinel ducks

UR - http://www.scopus.com/inward/record.url?scp=84879784330&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2013.05.004

DO - 10.1016/j.virol.2013.05.004

M3 - Article

C2 - 23725694

AN - SCOPUS:84879784330

VL - 443

SP - 150

EP - 160

JO - Virology

JF - Virology

SN - 1096-0341

IS - 1

ER -