FSP27 and PLIN1 interaction promotes the formation of large lipid droplets in human adipocytes.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Human adipocytes express high levels of two distinct lipid droplet proteins, fat specific protein 27 (FSP27; also called CIDEC), a member of the CIDE family, and perilipin1 (PLIN1), a member of the PAT family. Both proteins play a role in fat metabolism in adipocytes, but how they interact is not known. Our present study demonstrates that FSP27 and PLIN1 co-localize and interact in cultured human primary adipocytes. We also found that the C-terminal domain of FSP27, aa 120-220, interacts with PLIN1. Individual expression of exogenous FSP27 or PLIN1 increased triglyceride content and decreased glycerol release (a measure of lipolysis), but co-expression of both proteins did not further increase triglyceride content or decrease lipolysis in human adipocytes. However, the combination of PLIN1 and FSP27 increased the average size of lipid droplets or caused the formation of unilocular adipocytes. Our data suggest that FSP27 interacts with PLIN1 to regulate lipid droplet size in human adipocytes in a concerted manner.

Detaljer

Författare
  • Hooi Min Tan Grahn
  • Yan Zhang
  • Mi-Jeong Lee
  • Andreia Gianotti Sommer
  • Gustavo Mostoslavsky
  • Susan K Fried
  • Andrew S Greenberg
  • Vishwajeet Puri
Externa organisationer
  • External Organization - Unknown
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)296-301
TidskriftBiochemical and Biophysical Research Communications
Volym432
Utgivningsnummer2
StatusPublished - 2013
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa