Functional state of the β cell affects expression of both forms of glutamic acid decarboxylase

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title = "Functional state of the β cell affects expression of both forms of glutamic acid decarboxylase",
abstract = "Glutamic acid decarboxylase (GAD) is a candidate target autoantigen involved in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). The functional state of the B cells has been suggested to play a pathogenic role in IDDM by altering β-cell autoantigen expression. In this study, we investigated expression of GAD-65 and GAD-67 in isolated Sprague-Dawley rat islets cultured at different glucose concentrations. Using GAD isoform-specific antibodies in an immunoblot assay, we found that expression of both GAD-65 and GAD-67 in cultured islets was glucose dependent and that increased expression of both forms of GAD correlated with increased functional state of the β cell. Our data indicate that the functional state of the β cell influences islet cell expression of GAD. Thus, decreasing islet cell expression of GAD by suppressing β cells activity may have a potential role in blunting the autoimmune destruction of pancreatic islet B cells.",
keywords = "Glutamic acid decarboxylase, Insulin-dependent diabetes mellitus, β cell function",
author = "Wei Hao and Linsong Li and Vivek Mehta and {\AA}ke Lernmark and Palmer, {Jerry P.}",
year = "1994",
month = jan,
day = "1",
doi = "10.1097/00006676-199409000-00003",
language = "English",
volume = "9",
pages = "558--562",
journal = "Pancreas",
issn = "0885-3177",
publisher = "Lippincott Williams & Wilkins",
number = "5",