Functional state of the β cell affects expression of both forms of glutamic acid decarboxylase

TY - JOUR

T1 - Functional state of the β cell affects expression of both forms of glutamic acid decarboxylase

AU - Hao, Wei

AU - Li, Linsong

AU - Mehta, Vivek

AU - Lernmark, Åke

AU - Palmer, Jerry P.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Glutamic acid decarboxylase (GAD) is a candidate target autoantigen involved in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). The functional state of the B cells has been suggested to play a pathogenic role in IDDM by altering β-cell autoantigen expression. In this study, we investigated expression of GAD-65 and GAD-67 in isolated Sprague-Dawley rat islets cultured at different glucose concentrations. Using GAD isoform-specific antibodies in an immunoblot assay, we found that expression of both GAD-65 and GAD-67 in cultured islets was glucose dependent and that increased expression of both forms of GAD correlated with increased functional state of the β cell. Our data indicate that the functional state of the β cell influences islet cell expression of GAD. Thus, decreasing islet cell expression of GAD by suppressing β cells activity may have a potential role in blunting the autoimmune destruction of pancreatic islet B cells.

AB - Glutamic acid decarboxylase (GAD) is a candidate target autoantigen involved in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). The functional state of the B cells has been suggested to play a pathogenic role in IDDM by altering β-cell autoantigen expression. In this study, we investigated expression of GAD-65 and GAD-67 in isolated Sprague-Dawley rat islets cultured at different glucose concentrations. Using GAD isoform-specific antibodies in an immunoblot assay, we found that expression of both GAD-65 and GAD-67 in cultured islets was glucose dependent and that increased expression of both forms of GAD correlated with increased functional state of the β cell. Our data indicate that the functional state of the β cell influences islet cell expression of GAD. Thus, decreasing islet cell expression of GAD by suppressing β cells activity may have a potential role in blunting the autoimmune destruction of pancreatic islet B cells.

KW - Glutamic acid decarboxylase

KW - Insulin-dependent diabetes mellitus

KW - β cell function

UR - http://www.scopus.com/inward/record.url?scp=0028070978&partnerID=8YFLogxK

U2 - 10.1097/00006676-199409000-00003

DO - 10.1097/00006676-199409000-00003

M3 - Article

C2 - 7809009

AN - SCOPUS:0028070978

VL - 9

SP - 558

EP - 562

JO - Pancreas

JF - Pancreas

SN - 0885-3177

IS - 5

ER -