Functionally involved cell surface antigens on murine lymphokine activated killer cells

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

To investigate the possible involvement of some cell surface structures on lymphoid cells in the functional activity of lymphokine activated killer (LAK) cells, a number of monoclonal antibodies (Mab) against such structures was studied for their ability to inhibit LAK activity in a standard cytotoxicity assay against the natural killer-insensitive target cell EL-4. Almost complete inhibition of LAK activity resulted from incubation with antibodies to the LFA-1 antigen, while blocking of the Lyt 2 antigen reduced cytotoxic activity about 50%. Mab to T-200 gave a weak and inconsistent inhibitory activity, while antibodies to Thy 1, L3T4, IL-2 receptor and MHC class I antigens were without effect. Mab to LFA-1 and Lyt 2 inhibited LAK activity towards EL-4, YAC-1 and differentiated F-9 teratocarcinoma cells, but did not affect LAK-mediated killing of undifferentiated F-9 cells. Experiments with separate preincubation of effector and target cells revealed that both LFA-1 and Lyt 2 inhibited LAK activity at the effector cell level only.

Detaljer

Författare
  • Hans Belfrage
  • Pallonji Bhiladvala
  • T Kalland
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Medicinska grundvetenskaper
Originalspråkengelska
Sidor (från-till)293-8
TidskriftImmunology Letters
Volym14
Utgivningsnummer4
StatusPublished - 1987
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Belfrage, H., 1996, Department of Cell and Molecular Biology, Lund University. 58 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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