Functionally involved cell surface antigens on murine lymphokine activated killer cells
Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift
To investigate the possible involvement of some cell surface structures on lymphoid cells in the functional activity of lymphokine activated killer (LAK) cells, a number of monoclonal antibodies (Mab) against such structures was studied for their ability to inhibit LAK activity in a standard cytotoxicity assay against the natural killer-insensitive target cell EL-4. Almost complete inhibition of LAK activity resulted from incubation with antibodies to the LFA-1 antigen, while blocking of the Lyt 2 antigen reduced cytotoxic activity about 50%. Mab to T-200 gave a weak and inconsistent inhibitory activity, while antibodies to Thy 1, L3T4, IL-2 receptor and MHC class I antigens were without effect. Mab to LFA-1 and Lyt 2 inhibited LAK activity towards EL-4, YAC-1 and differentiated F-9 teratocarcinoma cells, but did not affect LAK-mediated killing of undifferentiated F-9 cells. Experiments with separate preincubation of effector and target cells revealed that both LFA-1 and Lyt 2 inhibited LAK activity at the effector cell level only.
|Enheter & grupper|
Ämnesklassifikation (UKÄ) – OBLIGATORISK
|Status||Published - 1987|
|Peer review utförd||Ja|
Activation of murine cytotoxic cells with interelukin-2 and the bacterial superantigen staphylococcal entertoxin A.Belfrage, H., 1996, Department of Cell and Molecular Biology, Lund University. 58 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)