Gastrointestinal conditions influence the solution behaviour of cereal beta-glucans in vitro

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The solution behaviour of b-glucans in a gastrointestinal model was investigated in order to explore the mechanisms explaining the physiological effects of the soluble fibre. Asymmetrical flow field-flow fractionation was used to determine the molar mass distribution, and in-line calcofluor labelling allowed specific
detection of b-glucans in complex samples. When dispersed in water, weight-average molar mass (Mw) was determined to 1 * 10^6 g/mol for pure oat and barley b-glucans, and 200 * 10^6 g/mol for b-glucans
in oat bran, indicating that the b-glucans were aggregating. Samples from the gastric digestion displayed disrupted aggregates, while samples from the small intestinal digestion contained re-formed
aggregates. Additionally, the aggregates from pure b-glucans were considerably denser after intestinal digestion. This may be construed as gel-formation in the small intestine, which should be tested for its relevance to health effects. Our results signal the difficulties in predicting b-glucan activity in the gastrointestinal tract purely from analysis of the fibre-rich product.


Enheter & grupper

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Livsmedelsteknik
  • Biokemi och molekylärbiologi


Sidor (från-till)536-540
TidskriftFood Chemistry
StatusPublished - 2012
Peer review utfördJa