Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury

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Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury. / Kapur, Rick; Kim, Michael; Rebetz, Johan; Hallström, Björn; Björkman, Jonas T; Takabe-French, Alisa; Kim, Noel; Liu, Jonathan; Shanmugabhavananthan, Shanjeevan; Milosevic, Stefan; McVey, Mark J; Speck, Edwin R; Semple, John W.

I: Blood Advances, Vol. 2, Nr. 13, 10.07.2018, s. 1651-1663.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Kapur, R, Kim, M, Rebetz, J, Hallström, B, Björkman, JT, Takabe-French, A, Kim, N, Liu, J, Shanmugabhavananthan, S, Milosevic, S, McVey, MJ, Speck, ER & Semple, JW 2018, 'Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury', Blood Advances, vol. 2, nr. 13, s. 1651-1663. https://doi.org/10.1182/bloodadvances.2018018903

APA

CBE

Kapur R, Kim M, Rebetz J, Hallström B, Björkman JT, Takabe-French A, Kim N, Liu J, Shanmugabhavananthan S, Milosevic S, McVey MJ, Speck ER, Semple JW. 2018. Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury. Blood Advances. 2(13):1651-1663. https://doi.org/10.1182/bloodadvances.2018018903

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Author

Kapur, Rick ; Kim, Michael ; Rebetz, Johan ; Hallström, Björn ; Björkman, Jonas T ; Takabe-French, Alisa ; Kim, Noel ; Liu, Jonathan ; Shanmugabhavananthan, Shanjeevan ; Milosevic, Stefan ; McVey, Mark J ; Speck, Edwin R ; Semple, John W. / Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury. I: Blood Advances. 2018 ; Vol. 2, Nr. 13. s. 1651-1663.

RIS

TY - JOUR

T1 - Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury

AU - Kapur, Rick

AU - Kim, Michael

AU - Rebetz, Johan

AU - Hallström, Björn

AU - Björkman, Jonas T

AU - Takabe-French, Alisa

AU - Kim, Noel

AU - Liu, Jonathan

AU - Shanmugabhavananthan, Shanjeevan

AU - Milosevic, Stefan

AU - McVey, Mark J

AU - Speck, Edwin R

AU - Semple, John W

N1 - © 2018 by The American Society of Hematology.

PY - 2018/7/10

Y1 - 2018/7/10

N2 - Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.

AB - Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.

U2 - 10.1182/bloodadvances.2018018903

DO - 10.1182/bloodadvances.2018018903

M3 - Article

VL - 2

SP - 1651

EP - 1663

JO - Blood Advances

T2 - Blood Advances

JF - Blood Advances

SN - 2473-9529

IS - 13

ER -