Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene

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Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene. / Marote, Ana; Pomeshchik, Yuriy; Collin, Anna; Goldwurm, Stefano; Lamas, Nuno J.; Pinto, Luísa; Salgado, António J.; Roybon, Laurent.

I: Stem Cell Research, Vol. 28, 01.04.2018, s. 44-47.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Marote, Ana ; Pomeshchik, Yuriy ; Collin, Anna ; Goldwurm, Stefano ; Lamas, Nuno J. ; Pinto, Luísa ; Salgado, António J. ; Roybon, Laurent. / Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene. I: Stem Cell Research. 2018 ; Vol. 28. s. 44-47.

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TY - JOUR

T1 - Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene

AU - Marote, Ana

AU - Pomeshchik, Yuriy

AU - Collin, Anna

AU - Goldwurm, Stefano

AU - Lamas, Nuno J.

AU - Pinto, Luísa

AU - Salgado, António J.

AU - Roybon, Laurent

PY - 2018/4/1

Y1 - 2018/4/1

N2 - The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD.

AB - The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD.

U2 - 10.1016/j.scr.2018.01.022

DO - 10.1016/j.scr.2018.01.022

M3 - Article

VL - 28

SP - 44

EP - 47

JO - Stem Cell Research

T2 - Stem Cell Research

JF - Stem Cell Research

SN - 1876-7753

ER -