Genomics, transcriptomics, and peptidomics of Daphnia pulex neuropeptides and protein hormones

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR. MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, allatotropin, Ala(7)-CCAP, CCHamide, Arg(7)-corazonin, DENamides, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin, two alternative splice forms of ion transport peptide (ITP), myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerokinins (but no pyrokinins), pigment dispersing hormone, proctolin, Met(4)-proctolin, short neuropeptide-F, three RYamides, SIFamide, two sulfakinins, and three tachykinins. There are two genes for a preprohormone containing orcomyotropin-like peptides and orcokinins, two genes for N-terminally elongated ITPs, two genes (clustered) for eclosion hormones, two genes (clustered) for bursicons alpha, beta, and two genes (clustered) for glycoproteins GPA2, GPB5, three genes for different allatostatins-C (two of them clustered) and three genes for IGF-related peptides. Detailed comparisons of genes or their products with those from insects and decapod crustaceans revealed that the D. pulex peptides are often closer related to their insect than to their decapod crustacean homologues, confirming that branchiopods, to which Daphnia belongs, are the ancestor group of insects.

Detaljer

Författare
  • Heinrich Dircksen
  • Susanne Neupert
  • Reinhard Predel
  • Peter Verleyen
  • Jurgen Huybrechts
  • Johannes Strauss
  • Frank Hauser
  • Elisabeth Stafflinger
  • Martina Schneider
  • Kevin Pauwels
  • Liliane Schoofs
  • Cornelis J P Grimmelikhuijzen
Externa organisationer
  • University of Copenhagen
  • Stockholms universitet
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)4478-4504
TidskriftJournal of Proteome Research
Volym10
Utgivningsnummer10
StatusPublished - 2011 okt 7
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa