Glioma cell activation by Alzheimer's peptide Abeta1-42, alpha1-antichymotrypsin, and their mixture.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We compared the effects of Alzheimer’s peptide
(Ab1–42), a1-antichymotrypsin (ACT) and an ACT/Ab1–42
mixture on human glioma DK-MG cells. The solution of
Ab (5 mM) formed by 2-h incubation at room temperature
induced tumour necrosis factor-a (TNF-a) and interleukin
(IL)-6 levels by 55 and 45%, respectively, and increased
gelatinase B activity by 67%, while exposure of
cells to the ACT/Ab1–42 mixture (1:10 molar ratio ACT:
Ab1–42) under the same experimental conditions showed
no effect on IL-6 levels or gelatinase B activity, but
strongly induced TNF-a (by 190%), compared to the con-
CMLS, Cell. Mol. Life Sci. 59 (2002) 1734–1743
1420-682X/02/101734-11
© Birkhäuser Verlag, Basel, 2002 CMLS Cellular and Molecular Life Sciences
trols. Stimulation of the cells with Ab1–42 alone, but not
with ACT, increased by about 20% low-density lipoprotein
(LDL) uptake and mRNA levels for LDL receptor and
HMG-CoA reductase, while the ACT/Ab1–42 mixture significantly
increased LDL uptake (by 50%), up-regulated
mRNA levels for LDL receptor and HMG-CoA reductase
by 48 and 63%, respectively, and increased lipid accumulation
by about 20-fold. These data suggest a possible new
role for Ab in Alzheimer’s disease through its interaction
with the inflammatory reactant, ACT.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cellbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)1734-43
TidskriftCellular and Molecular Life Sciences
Volym59
Utgivningsnummer10
StatusPublished - 2002
PublikationskategoriForskning
Peer review utfördJa