Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Understanding how a pathogen colonizes and adapts to a new host environment is a primary aim in studying emerging infectious diseases. Adaptive mutations arise among the thousands of variants generated during RNA virus infection, and identifying these variants will shed light onto how changes in tropism and species jumps can occur. Here, we adapted Coxsackie virus B3 to a highly permissive and less permissive environment. Using deep sequencing and bioinformatics, we identified a multi-step adaptive process to adaptation involving residues in the receptor footprints that correlated with receptor availability and with increase in virus fitness in an environment-specific manner. We show that adaptation occurs by selection of a dominant mutation followed by group selection of minority variants that together, confer the fitness increase observed in the population, rather than selection of a single dominant genotype.

Detaljer

Författare
  • Antonio V Bordería
  • Ofer Isakov
  • Gonzalo Moratorio
  • Rasmus Henningsson
  • Sonia Agüera-González
  • Lindsey Organtini
  • Nina F Gnädig
  • Hervé Blanc
  • Andrés Alcover
  • Susan Hafenstein
  • Magnus Fontes
  • Noam Shomron
  • Marco Vignuzzi
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Immunologi
Originalspråkengelska
Artikelnummere1004838
TidskriftPLoS Pathogens
Volym11
Utgivningsnummer5
StatusPublished - 2015
PublikationskategoriForskning
Peer review utfördJa