Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKapitel samlingsverk

Abstract

It is estimated that over 80% of bacterial infections are associated with biofilm formation. Biofilms are organized bacterial communities formed on abiotic surfaces, such as implanted or inserted medical devices, or on biological surfaces, such as epithelial linings and mucosal surfaces. Biofilm growth is advantageous for the bacterial organism as it protects the bacteria from antimicrobial host factors and allows the bacteria to reside in the host without causing excessive inflammation. Like many other opportunistic pathogens of the respiratory tract, Streptococcus pneumoniae forms biofilms during asymptomatic carriage, which promotes, among other things, persistence in the niche, intraspecies and interspecies communication, and spread of bacterial DNA. Changes within the colonizing environment resulting from host assaults, such as virus infection, can induce biofilm dispersion where bacteria leave the biofilm and disseminate to other sites with ensuing infection. In this chapter, we present methodology to form complex biofilms in the nasopharynx of mice and to evaluate the biofilm structure and function in this environment. Furthermore, we present methods that recapitulate this biofilm phenotype in vitro by incorporating crucial factors associated with the host environment and describe how these models can be used to study biofilm function, transformation, and dispersion.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Infektionsmedicin
Originalspråkengelska
Titel på värdpublikationStreptococcus pneumoniae
Undertitel på gästpublikationMethods and Protocols
FörlagHumana Press
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Sidor147-171
Volym1968
ISBN (elektroniskt)978-1-4939-9199-0
ISBN (tryckt)978-1-4939-9198-3
StatusPublished - 2019
PublikationskategoriForskning
Peer review utfördJa

Publikationsserier

NamnMethods in molecular biology (Clifton, N.J.)
FörlagSpringer
ISSN (tryckt)1940-6029

Relaterad forskningsoutput

Yashuan Chao, 2019, Lund: Lund University, Faculty of Medicine. 45 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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