Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study

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Abstract

Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.

Detaljer

Författare
  • Mari C.W. Myhrstad
  • Vanessa D. de Mello
  • Ingrid Dahlman
  • Marjukka Kolehmainen
  • Jussi Paananen
  • Amanda Rundblad
  • Carsten Carlberg
  • Ole Kristoffer Olstad
  • Jussi Pihlajamäki
  • Kirsten B. Holven
  • Kjeld Hermansen
  • Lars O. Dragsted
  • Ingibjörg Gunnarsdottir
  • Matilda Ulmius Storm
  • Fredrik Rosqvist
  • Janne Hukkanen
  • Karl Heinz Herzig
  • Ulf Risérus
  • Inga Thorsdottir
  • Kaisa S. Poutanen
  • Markku J. Savolainen
  • Ursula Schwab
  • Peter Arner
  • Matti Uusitupa
  • Stine M. Ulven
Enheter & grupper
Externa organisationer
  • University of Eastern Finland
  • Karolinska Institute
  • University of Oslo
  • Oslo university hospital
  • Kuopio University Hospital
  • Aarhus University Hospital
  • University of Copenhagen
  • National University Hospital of Iceland
  • Skåne University Hospital
  • Uppsala universitet, Historiska institutionen
  • University of Oulu
  • Poznan University of Medical Sciences
  • VTT Technical Research Centre of Finland Ltd
  • Oslo Metropolitan University
  • University of Iceland
  • Oulu University Hospital
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Näringslära

Nyckelord

Originalspråkengelska
Artikelnummer1801405
TidskriftMolecular Nutrition and Food Research
StatusE-pub ahead of print - 2019 apr 9
PublikationskategoriForskning
Peer review utfördJa