Hepatic Leukemia Factor Maintains Quiescence of Hematopoietic Stem Cells and Protects the Stem Cell Pool during Regeneration

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The transcription factor hepatic leukemia factor (HLF) is strongly expressed in hematopoietic stem cells (HSCs) and is thought to influence both HSC self-renewal and leukemogenesis. However, the physiological role of HLF in hematopoiesis and HSC function is unclear. Here, we report that mice lacking Hlf are viable with essentially normal hematopoietic parameters, including an intact HSC pool during steady-state hematopoiesis. In contrast, when challenged through transplantation, Hlf-deficient HSCs showed an impaired ability to reconstitute hematopoiesis and became gradually exhausted upon serial transplantation. Transcriptional profiling of Hlf-deficient HSCs revealed changes associated with enhanced cellular activation, and cell-cycle analysis demonstrated a significant reduction of quiescent HSCs. Accordingly, toxic insults targeting dividing cells completely eradicated the HSC pool in Hlf-deficient mice. In summary, our findings point to HLF as a critical regulator of HSC quiescence and as an essential factor for maintaining the HSC pool during regeneration. Komorowska et al. report that the transcription factor HLF is required to maintain hematopoietic stem cell (HSC) function during regeneration. Moreover, Hlf-deficient HSCs are less quiescent. In accordance with this, toxic insults targeting dividing cells completely eradicate the HSC pool in Hlf-deficient mice.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • University of Southern California
  • University of California, Los Angeles
  • CarthroniX Inc
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Hematologi
  • Cell- och molekylärbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)3514-3523
Antal sidor10
TidskriftCell Reports
Volym21
Utgivningsnummer12
StatusPublished - 2017 dec 19
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Komorowska, K., 2018, Lund: Lund University, Faculty of Medicine. 100 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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