HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Farmakologi och toxikologi
  • Läkemedelskemi
Originalspråkengelska
Sidor (från-till)297-307
TidskriftMethods in Molecular Biology
Volym919
StatusPublished - 2013
PublikationskategoriForskning
Peer review utfördJa