HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses

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HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses. / Rollman, E; Arnheim, L; Collier, B; Oberg, D; Hall, H; Klingstrom, J; Dillner, Joakim; Pastrana, DV; Buck, CB; Hinkula, J; Wahren, B; Schwartz, S.

I: Virology, Vol. 322, Nr. 1, 2004, s. 182-189.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Rollman, E, Arnheim, L, Collier, B, Oberg, D, Hall, H, Klingstrom, J, Dillner, J, Pastrana, DV, Buck, CB, Hinkula, J, Wahren, B & Schwartz, S 2004, 'HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses', Virology, vol. 322, nr. 1, s. 182-189. https://doi.org/10.1016/j.virol.2004.02.002

APA

Rollman, E., Arnheim, L., Collier, B., Oberg, D., Hall, H., Klingstrom, J., Dillner, J., Pastrana, DV., Buck, CB., Hinkula, J., Wahren, B., & Schwartz, S. (2004). HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses. Virology, 322(1), 182-189. https://doi.org/10.1016/j.virol.2004.02.002

CBE

Rollman E, Arnheim L, Collier B, Oberg D, Hall H, Klingstrom J, Dillner J, Pastrana DV, Buck CB, Hinkula J, Wahren B, Schwartz S. 2004. HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses. Virology. 322(1):182-189. https://doi.org/10.1016/j.virol.2004.02.002

MLA

Vancouver

Rollman E, Arnheim L, Collier B, Oberg D, Hall H, Klingstrom J et al. HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses. Virology. 2004;322(1):182-189. https://doi.org/10.1016/j.virol.2004.02.002

Author

Rollman, E ; Arnheim, L ; Collier, B ; Oberg, D ; Hall, H ; Klingstrom, J ; Dillner, Joakim ; Pastrana, DV ; Buck, CB ; Hinkula, J ; Wahren, B ; Schwartz, S. / HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses. I: Virology. 2004 ; Vol. 322, Nr. 1. s. 182-189.

RIS

TY - JOUR

T1 - HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses

AU - Rollman, E

AU - Arnheim, L

AU - Collier, B

AU - Oberg, D

AU - Hall, H

AU - Klingstrom, J

AU - Dillner, Joakim

AU - Pastrana, DV

AU - Buck, CB

AU - Hinkula, J

AU - Wahren, B

AU - Schwartz, S

PY - 2004

Y1 - 2004

N2 - Introduction of point mutations in the 5' end of the human papillomavirus type 16 (HPV-16) L1 gene specifically inactivates negative regulatory RNA processing elements. DNA vaccination of C57B1/6 mice with the mutated L1 gene resulted in improved immunogenicity for both neutralizing antibodies as well as for broad cellular immune responses. Previous reports on the activation of L1 by codon optimization may be explained by inactivation of the regulatory RNA elements. The modified HPV-16 L1 DNA that induced anti-HPV-16 immunity may be seen as a complementary approach to protein subunit immunization against papillomavirus.

AB - Introduction of point mutations in the 5' end of the human papillomavirus type 16 (HPV-16) L1 gene specifically inactivates negative regulatory RNA processing elements. DNA vaccination of C57B1/6 mice with the mutated L1 gene resulted in improved immunogenicity for both neutralizing antibodies as well as for broad cellular immune responses. Previous reports on the activation of L1 by codon optimization may be explained by inactivation of the regulatory RNA elements. The modified HPV-16 L1 DNA that induced anti-HPV-16 immunity may be seen as a complementary approach to protein subunit immunization against papillomavirus.

KW - imiquimod

KW - rGM-CSF

KW - T lymphocytes

KW - neutralization

KW - tumor immunity

KW - DNA vaccination

KW - HPV-16

U2 - 10.1016/j.virol.2004.02.002

DO - 10.1016/j.virol.2004.02.002

M3 - Article

C2 - 15063127

VL - 322

SP - 182

EP - 189

JO - Virology

JF - Virology

SN - 1096-0341

IS - 1

ER -