Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine

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Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine. / Barregard, Lars; Moller, Peter; Henriksen, Trine; Mistry, Vilas; Koppen, Gudrun; Rossner Jr., Pavel; Sram, Radim J.; Weimann, Allan; Poulsen, Henrik E.; Nataf, Robert; Andreoli, Roberta; Manini, Paola; Marczylo, Tim; Lam, Patricia; Evans, Mark D.; Kasai, Hiroshi; Kawai, Kazuaki; Li, Yun-Shan; Sakai, Kazuo; Singh, Rajinder; Teichert, Friederike; Farmer, Peter B.; Rozalski, Rafal; Gackowski, Daniel; Siomek, Agnieszka; Saez, Guillermo T.; Cerda, Concha; Broberg Palmgren, Karin; Lindh, Christian; Hossain, Bakhtiar; Haghdoost, Siamak; Hu, Chiung-Wen; Chao, Mu-Rong; Wu, Kuen-Yuh; Orhan, Hilmi; Senduran, Nilufer; Smith, Raymond J.; Santella, Regina M.; Su, Yali; Cortez, Czarina; Yeh, Susan; Olinski, Ryszard; Loft, Steffen; Cooke, Marcus S.

I: Antioxidants & Redox Signaling, Vol. 18, Nr. 18, 2013, s. 2377-2391.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Barregard, L, Moller, P, Henriksen, T, Mistry, V, Koppen, G, Rossner Jr., P, Sram, RJ, Weimann, A, Poulsen, HE, Nataf, R, Andreoli, R, Manini, P, Marczylo, T, Lam, P, Evans, MD, Kasai, H, Kawai, K, Li, Y-S, Sakai, K, Singh, R, Teichert, F, Farmer, PB, Rozalski, R, Gackowski, D, Siomek, A, Saez, GT, Cerda, C, Broberg Palmgren, K, Lindh, C, Hossain, B, Haghdoost, S, Hu, C-W, Chao, M-R, Wu, K-Y, Orhan, H, Senduran, N, Smith, RJ, Santella, RM, Su, Y, Cortez, C, Yeh, S, Olinski, R, Loft, S & Cooke, MS 2013, 'Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine', Antioxidants & Redox Signaling, vol. 18, nr. 18, s. 2377-2391. https://doi.org/10.1089/ars.2012.4714

APA

Barregard, L., Moller, P., Henriksen, T., Mistry, V., Koppen, G., Rossner Jr., P., Sram, R. J., Weimann, A., Poulsen, H. E., Nataf, R., Andreoli, R., Manini, P., Marczylo, T., Lam, P., Evans, M. D., Kasai, H., Kawai, K., Li, Y-S., Sakai, K., ... Cooke, M. S. (2013). Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine. Antioxidants & Redox Signaling, 18(18), 2377-2391. https://doi.org/10.1089/ars.2012.4714

CBE

Barregard L, Moller P, Henriksen T, Mistry V, Koppen G, Rossner Jr. P, Sram RJ, Weimann A, Poulsen HE, Nataf R, Andreoli R, Manini P, Marczylo T, Lam P, Evans MD, Kasai H, Kawai K, Li Y-S, Sakai K, Singh R, Teichert F, Farmer PB, Rozalski R, Gackowski D, Siomek A, Saez GT, Cerda C, Broberg Palmgren K, Lindh C, Hossain B, Haghdoost S, Hu C-W, Chao M-R, Wu K-Y, Orhan H, Senduran N, Smith RJ, Santella RM, Su Y, Cortez C, Yeh S, Olinski R, Loft S, Cooke MS. 2013. Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine. Antioxidants & Redox Signaling. 18(18):2377-2391. https://doi.org/10.1089/ars.2012.4714

MLA

Vancouver

Author

Barregard, Lars ; Moller, Peter ; Henriksen, Trine ; Mistry, Vilas ; Koppen, Gudrun ; Rossner Jr., Pavel ; Sram, Radim J. ; Weimann, Allan ; Poulsen, Henrik E. ; Nataf, Robert ; Andreoli, Roberta ; Manini, Paola ; Marczylo, Tim ; Lam, Patricia ; Evans, Mark D. ; Kasai, Hiroshi ; Kawai, Kazuaki ; Li, Yun-Shan ; Sakai, Kazuo ; Singh, Rajinder ; Teichert, Friederike ; Farmer, Peter B. ; Rozalski, Rafal ; Gackowski, Daniel ; Siomek, Agnieszka ; Saez, Guillermo T. ; Cerda, Concha ; Broberg Palmgren, Karin ; Lindh, Christian ; Hossain, Bakhtiar ; Haghdoost, Siamak ; Hu, Chiung-Wen ; Chao, Mu-Rong ; Wu, Kuen-Yuh ; Orhan, Hilmi ; Senduran, Nilufer ; Smith, Raymond J. ; Santella, Regina M. ; Su, Yali ; Cortez, Czarina ; Yeh, Susan ; Olinski, Ryszard ; Loft, Steffen ; Cooke, Marcus S. / Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine. I: Antioxidants & Redox Signaling. 2013 ; Vol. 18, Nr. 18. s. 2377-2391.

RIS

TY - JOUR

T1 - Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine

AU - Barregard, Lars

AU - Moller, Peter

AU - Henriksen, Trine

AU - Mistry, Vilas

AU - Koppen, Gudrun

AU - Rossner Jr., Pavel

AU - Sram, Radim J.

AU - Weimann, Allan

AU - Poulsen, Henrik E.

AU - Nataf, Robert

AU - Andreoli, Roberta

AU - Manini, Paola

AU - Marczylo, Tim

AU - Lam, Patricia

AU - Evans, Mark D.

AU - Kasai, Hiroshi

AU - Kawai, Kazuaki

AU - Li, Yun-Shan

AU - Sakai, Kazuo

AU - Singh, Rajinder

AU - Teichert, Friederike

AU - Farmer, Peter B.

AU - Rozalski, Rafal

AU - Gackowski, Daniel

AU - Siomek, Agnieszka

AU - Saez, Guillermo T.

AU - Cerda, Concha

AU - Broberg Palmgren, Karin

AU - Lindh, Christian

AU - Hossain, Bakhtiar

AU - Haghdoost, Siamak

AU - Hu, Chiung-Wen

AU - Chao, Mu-Rong

AU - Wu, Kuen-Yuh

AU - Orhan, Hilmi

AU - Senduran, Nilufer

AU - Smith, Raymond J.

AU - Santella, Regina M.

AU - Su, Yali

AU - Cortez, Czarina

AU - Yeh, Susan

AU - Olinski, Ryszard

AU - Loft, Steffen

AU - Cooke, Marcus S.

PY - 2013

Y1 - 2013

N2 - Aims: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability. Results: Chromatographic assays showed high agreement across urines from different subjects, whereas ELISAs showed far more inter-laboratory variation and generally overestimated levels, compared to the chromatographic assays. Excretion rates in timed 'spot' samples showed strong correlations with 24 h excretion (the 'gold' standard) of urinary 8-oxodG (r(p) 0.67-0.90), although the associations were weaker for 8-oxodG adjusted for creatinine or specific gravity (SG). The within-individual excretion of 8-oxodG varied only moderately between days (CV 17% for 24 h excretion and 20% for first void, creatinine-corrected samples). Innovation: This is the first comprehensive study of both human and methodological factors influencing 8-oxodG measurement, providing key information for future studies with this important biomarker. Conclusion: ELISA variability is greater than chromatographic assay variability, and cannot determine absolute levels of 8-oxodG. Use of standardized calibrants greatly improves intra-technique agreement and, for the chromatographic assays, importantly allows integration of results for pooled analyses. If 24 h samples are not feasible, creatinine- or SG-adjusted first morning samples are recommended.

AB - Aims: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability. Results: Chromatographic assays showed high agreement across urines from different subjects, whereas ELISAs showed far more inter-laboratory variation and generally overestimated levels, compared to the chromatographic assays. Excretion rates in timed 'spot' samples showed strong correlations with 24 h excretion (the 'gold' standard) of urinary 8-oxodG (r(p) 0.67-0.90), although the associations were weaker for 8-oxodG adjusted for creatinine or specific gravity (SG). The within-individual excretion of 8-oxodG varied only moderately between days (CV 17% for 24 h excretion and 20% for first void, creatinine-corrected samples). Innovation: This is the first comprehensive study of both human and methodological factors influencing 8-oxodG measurement, providing key information for future studies with this important biomarker. Conclusion: ELISA variability is greater than chromatographic assay variability, and cannot determine absolute levels of 8-oxodG. Use of standardized calibrants greatly improves intra-technique agreement and, for the chromatographic assays, importantly allows integration of results for pooled analyses. If 24 h samples are not feasible, creatinine- or SG-adjusted first morning samples are recommended.

U2 - 10.1089/ars.2012.4714

DO - 10.1089/ars.2012.4714

M3 - Article

C2 - 23198723

VL - 18

SP - 2377

EP - 2391

JO - Antioxidants and Redox Signaling

JF - Antioxidants and Redox Signaling

SN - 1557-7716

IS - 18

ER -