Hydrodynamic radius determination with asymmetrical flow field-flow fractionation using decaying cross-flows. Part II. Experimental evaluation.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In this study we investigate the effect of programmed cross-flows on the error in the hydrodynamic radii (r(h)) determination with asymmetrical flow field-flow fractionation (AsFlFFF). Three different standard polystyrene particles (nominal radii of 30 and 40 and 50nm) are fractionated with exponentially and linearly decaying cross-flows with different decay rates. Hydrodynamic radii are calculated according to retention theory including steric effects. Rapid decay is expected to give rise to systematic deviations in r(h) determination. The error in r(h) was found to be small when decay rates with half-lives longer than 6min were used, whereas steeper decays could give rise to errors as high as 16% of the particle size. The error is often explained in terms of secondary relaxation. However, comparisons show that experimental errors are significantly larger than what would be expected due to secondary relaxation, suggesting that other factors also have to be considered in order to fully understand deviations for rapidly decaying cross-flow.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Livsmedelsteknik
  • Fysikalisk kemi
Originalspråkengelska
Sidor (från-till)127-133
TidskriftJournal of chromatography. A
Volym1253
StatusPublished - 2012
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Magnusson, E., 2015, Department of Food Technology, Engineering and Nutrition, Lund University. 121 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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