Identification of a streptococcal octapeptide motif involved in acute rheumatic fever

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Identification of a streptococcal octapeptide motif involved in acute rheumatic fever. / Dinkla, Katrin; Nitsche-Schmitz, D. Patric; Barroso, Vanessa; Reissmann, Silvana; Linge, Helena; Frick, Inga-Maria; Rohde, Manfred; Chhatwal, Gursharan S.

I: Journal of Biological Chemistry, Vol. 282, Nr. 26, 2007, s. 18686-18693.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Dinkla, K, Nitsche-Schmitz, DP, Barroso, V, Reissmann, S, Linge, H, Frick, I-M, Rohde, M & Chhatwal, GS 2007, 'Identification of a streptococcal octapeptide motif involved in acute rheumatic fever', Journal of Biological Chemistry, vol. 282, nr. 26, s. 18686-18693. https://doi.org/10.1074/jbc.M701047200

APA

Dinkla, K., Nitsche-Schmitz, D. P., Barroso, V., Reissmann, S., Linge, H., Frick, I-M., Rohde, M., & Chhatwal, G. S. (2007). Identification of a streptococcal octapeptide motif involved in acute rheumatic fever. Journal of Biological Chemistry, 282(26), 18686-18693. https://doi.org/10.1074/jbc.M701047200

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Author

Dinkla, Katrin ; Nitsche-Schmitz, D. Patric ; Barroso, Vanessa ; Reissmann, Silvana ; Linge, Helena ; Frick, Inga-Maria ; Rohde, Manfred ; Chhatwal, Gursharan S. / Identification of a streptococcal octapeptide motif involved in acute rheumatic fever. I: Journal of Biological Chemistry. 2007 ; Vol. 282, Nr. 26. s. 18686-18693.

RIS

TY - JOUR

T1 - Identification of a streptococcal octapeptide motif involved in acute rheumatic fever

AU - Dinkla, Katrin

AU - Nitsche-Schmitz, D. Patric

AU - Barroso, Vanessa

AU - Reissmann, Silvana

AU - Linge, Helena

AU - Frick, Inga-Maria

AU - Rohde, Manfred

AU - Chhatwal, Gursharan S.

PY - 2007

Y1 - 2007

N2 - Acute rheumatic fever is a serious autoimmune sequela of pharyngitis caused by certain group A streptococci. One mechanism applied by streptococcal strains capable of causing acute rheumatic fever is formation of an autoantigenic complex with human collagen IV. In some geographic regions with a high incidence of acute rheumatic fever pharyngeal carriage of group C and group G streptococci prevails. Examination of such strains revealed the presence of M-like surface proteins that bind human collagen. Using a peptide array and recombinant proteins with targeted amino acid substitutions, we could demonstrate that formation of collagen complexes during streptococcal infections depends on an octapeptide motif, which is present in collagen binding M and M-like proteins of different beta-hemolytic streptococcal species. Mice immunized with streptococcal proteins that contain the collagen binding octapeptide motif developed high serum titers of anti-collagen antibodies. In sera of rheumatic fever patients such a collagen autoimmune response was accompanied by specific reactivity against the collagen-binding proteins, linking the observed effect to clinical cases. Taken together, the data demonstrate that the identified octapeptide motif through its action on collagen plays a crucial role in the pathogenesis of rheumatic fever. Eradication of streptococci that express proteins with the collagen binding motif appears advisable for controlling rheumatic fever.

AB - Acute rheumatic fever is a serious autoimmune sequela of pharyngitis caused by certain group A streptococci. One mechanism applied by streptococcal strains capable of causing acute rheumatic fever is formation of an autoantigenic complex with human collagen IV. In some geographic regions with a high incidence of acute rheumatic fever pharyngeal carriage of group C and group G streptococci prevails. Examination of such strains revealed the presence of M-like surface proteins that bind human collagen. Using a peptide array and recombinant proteins with targeted amino acid substitutions, we could demonstrate that formation of collagen complexes during streptococcal infections depends on an octapeptide motif, which is present in collagen binding M and M-like proteins of different beta-hemolytic streptococcal species. Mice immunized with streptococcal proteins that contain the collagen binding octapeptide motif developed high serum titers of anti-collagen antibodies. In sera of rheumatic fever patients such a collagen autoimmune response was accompanied by specific reactivity against the collagen-binding proteins, linking the observed effect to clinical cases. Taken together, the data demonstrate that the identified octapeptide motif through its action on collagen plays a crucial role in the pathogenesis of rheumatic fever. Eradication of streptococci that express proteins with the collagen binding motif appears advisable for controlling rheumatic fever.

U2 - 10.1074/jbc.M701047200

DO - 10.1074/jbc.M701047200

M3 - Article

VL - 282

SP - 18686

EP - 18693

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 1083-351X

IS - 26

ER -