Identification of Iridoid Glucoside Transporters in Catharanthus roseus

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme expression data and transporter specificity, we propose that several enzymes of the biosynthetic pathway are present in both IPAP and epidermis cells, and that the three transporters are responsible for transporting not only loganic acid, as previously proposed, but multiple intermediates. Identification of the iridoid glucoside-transporting CrNPFs is an important step toward understanding the complex orchestration of the seco-iridioid pathway.

Detaljer

Författare
  • Lars Bo Larsen
  • Victoria L Fuller
  • Jacob Pollier
  • Alex Van Moerkercke
  • Fabian Schweizer
  • Richard J. Payne
  • Maite Colinas
  • Sarah E O'Connor
  • Alain Goossens
  • Barbara A Halkier
Externa organisationer
  • Vlaams Instituut voor Biotechnologie
  • University of Copenhagen
  • Ghent University
  • John Innes Centre
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi
Originalspråkengelska
Sidor (från-till)1507-1518
Antal sidor12
TidskriftPlant and Cell Physiology
Volym58
Utgåva nummer9
StatusPublished - 2017 sep 1
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa