Identification of Novel Genetic Markers Associated with Clinical Phenotypes of Systemic Sclerosis through a Genome-Wide Association Strategy

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The aim of this study was to determine, through a genome-wide association study (GWAS), the genetic components contributing to different clinical sub-phenotypes of systemic sclerosis (SSc). We considered limited (IcSSc) and diffuse (dcSSc) cutaneous involvement, and the relationships with presence of the SSc-specific auto-antibodies, anti-centromere (ACA), and anti-topoisomerase I (ATA). Four GWAS cohorts, comprising 2,296 SSc patients and 5,171 healthy controls, were meta-analyzed looking for associations in the selected subgroups. Eighteen polymorphisms were further tested in nine independent cohorts comprising an additional 3,175 SSc patients and 4,971 controls. Conditional analysis for associated SNPs in the HLA region was performed to explore their independent association in antibody subgroups. Overall analysis showed that non-HLA polymorphism rs11642873 in IRF8 gene to be associated at GWAS level with lcSSc (P = 2.32x10(-12), OR = 0.75). Also, rs12540874 in GRB10 gene (P = 1.27 x 10(-6), OR = 1.15) and rs11047102 in SOX5 gene (P = 1.39x10(-7), OR = 1.36) showed a suggestive association with lcSSc and ACA subgroups respectively. In the HLA region, we observed highly associated allelic combinations in the HLA-DQB1 locus with ACA (P = 1.79x10(-61), OR = 2.48), in the HLA-DPA1/B1 loci with ATA (P = 4.57x10(-76), OR = 8.84), and in NOTCH4 with ACA P = 8.84x10(-21), OR = 0.55) and ATA (P = 1.14x10(-8), OR = 0.54). We have identified three new non-HLA genes (IRF8, GRB10, and SOX5) associated with SSc clinical and autoantibody subgroups. Within the HLA region, HLA-DQB1, HLA-DPA1/B1, and NOTCH4 associations with SSc are likely confined to specific auto-antibodies. These data emphasize the differential genetic components of subphenotypes of SSc.

Detaljer

Författare
  • Olga Gorlova
  • Jose-Ezequiel Martin
  • Blanca Rueda
  • Bobby P. C. Koeleman
  • Jun Ying
  • Maria Teruel
  • Lina-Marcela Diaz-Gallo
  • Jasper C. Broen
  • Madelon C. Vonk
  • Carmen P. Simeon
  • Behrooz Z. Alizadeh
  • Marieke J. H. Coenen
  • Alexandre E. Voskuyl
  • Annemie J. Schuerwegh
  • Piet L. C. M. van Riel
  • Marie Vanthuyne
  • Ruben van't Slot
  • Annet Italiaander
  • Roel A. Ophoff
  • Nicolas Hunzelmann
  • Vicente Fonollosa
  • Norberto Ortego-Centeno
  • Miguel A. Gonzalez-Gay
  • Francisco J. Garcia-Hernandez
  • Maria F. Gonzalez-Escribano
  • Paolo Airo
  • Jacob van Laar
  • Jane Worthington
  • Vanessa Smith
  • Filip de Keyser
  • Fredric Houssiau
  • Meng May Chee
  • Rajan Madhok
  • Paul G. Shiels
  • Rene Westhovens
  • Alexander Kreuter
  • Elfride de Baere
  • Torsten Witte
  • Leonid Padyukov
  • Annika Nordin
  • Raffaella Scorza
  • Claudio Lunardi
  • Benedicte A. Lie
  • Anna-Maria Hoffmann-Vold
  • Oyvind Palm
  • Paloma Garcia de la Pena
  • Patricia Carreira
  • John Varga
  • Monique Hinchcliff
  • Annette T. Lee
  • Pravitt Gourh
  • Christopher I. Amos
  • Frederick M. Wigley
  • Laura K. Hummers
  • J. Hummers
  • J. Lee Nelson
  • Gabriella Riemekasten
  • Ariane Herrick
  • Lorenzo Beretta
  • Carmen Fonseca
  • Christopher P. Denton
  • Peter K. Gregersen
  • Sandeep Agarwal
  • Shervin Assassi
  • Filemon K. Tan
  • Frank C. Arnett
  • Timothy R. D. J. Radstake
  • Maureen D. Mayes
  • Javier Martin
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Reumatologi och inflammation
Originalspråkengelska
TidskriftPLoS Genetics
Volym7
Utgivningsnummer7
StatusPublished - 2011
PublikationskategoriForskning
Peer review utfördJa

Nedladdningar

Ingen tillgänglig data