Identification of novel proteolytic forms of osteocalcin in human urine

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In this study, we report the isolation and characterization of osteocalcin in human urine using mass spectrometry and N-terminal sequencing. Multiple proteolytic forms of osteocalcin were found, which consisted of 16-27 residues from the middle region of the molecule. Several fragments had residue Gly7 at the N-terminus and the most predominant was fragment 7-31. Additional fragments starting from residue Asp14 were detected in the samples of children and young adults. Immunochemical detection of urine osteocalcin fragments had a statistically significant negative correlation to bone mineral density in evaluation of urine samples from 75-year-old women. Thus, the measurement of osteocalcin fragments in urine may have potential applications in diagnostics related to disorders of bone metabolism.

Detaljer

Författare
  • KK Ivaska
  • J Hellman
  • J Likojarvi
  • SM Kakonen
  • Paul Gerdhem
  • Kristina Åkesson
  • Karl Obrant
  • K Pettersson
  • HK Vaananen
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper
Originalspråkengelska
Sidor (från-till)973-980
TidskriftBiochemical and Biophysical Research Communications
Volym306
Utgivningsnummer4
StatusPublished - 2003
PublikationskategoriForskning
Peer review utfördJa