Identification of the ligand of Pru p 3, a peach LTP

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Abstract

Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.

Detaljer

Författare
  • Nuria Cubells-Baeza
  • Cristina Gómez-Casado
  • Leticia Tordesillas
  • Carmen Ramírez-Castillejo
  • María Garrido-Arandia
  • Pablo González-Melendi
  • María Herrero
  • Luis F. Pacios
  • Araceli Díaz-Perales
Enheter & grupper
Externa organisationer
  • Technical University of Madrid
  • Centre for Plant Biotechnology and Genomics
  • Icahn School of Medicine at Mount Sinai
  • CSIC - Estacion Experimental de Aula Dei EEAD
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi
  • Botanik

Nyckelord

Originalspråkengelska
Sidor (från-till)33-44
Antal sidor12
TidskriftPlant Molecular Biology
Volym94
Utgåva nummer1-2
Tidigt onlinedatum2017 mar 15
StatusPublished - 2017 maj
PublikationskategoriForskning
Peer review utfördJa