Identifying bacteria using DNA binding maps

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKonferenspaper i proceeding

Abstract

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Chalmers University of Technology
  • Sahlgrenska Academy
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biofysik

Nyckelord

Originalspråkengelska
Titel på värdpublikation17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
FörlagChemical and Biological Microsystems Society
Sidor473-475
Antal sidor3
Volym1
ISBN (tryckt)9781632666246
StatusPublished - 2013 jan 1
PublikationskategoriForskning
Peer review utfördJa
Evenemang17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Tyskland
Varaktighet: 2013 okt 272013 okt 31

Konferens

Konferens17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
LandTyskland
OrtFreiburg
Period2013/10/272013/10/31