Immobilization to Positively Charged Cellulose Nanocrystals Enhances the Antibacterial Activity and Stability of Hen Egg White and T4 Lysozyme

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Antibacterial bionanostructures were produced from cellulose nanocrystals (CNC) with immobilized lysozyme from hen egg white (HEW) and T4 bacteriophage, respectively. The nanocrystals were prepared from microcrystalline cellulose by ammonium persulfate oxidation with a yield of 68% and having an average size of 250 nm and low polydispersity index. HEW lysozyme (HEWL) and T4 lysozyme (T4L) were immobilized to CNC by different mechanisms including adsorption and covalent coupling to carbodiimide-activated carboxylate groups and to glutaraldehyde-activated aminated CNC (Am-CNC), respectively. The effect of immobilization on the enzymatic activity (both lytic and hydrolytic) and antibacterial activity of the lysozymes was studied using different methods. Am-CNC-lysozyme conjugates retained the highest lytic activity, 86.3% and 78.3% for HEWL and T4L, respectively. They also showed enhanced bactericidal activity with high potency against Gram-positive as well as Gram-negative bacteria in a relatively shorter time as compared to the free enzymes and resulted in extensive cellular damage, as shown by transmission electron microscopy. The enhanced antibacterial activity was correlated with the increase in zeta potential of Am-CNC-lysozyme conjugates. The immobilized lysozyme preparations further exhibited enhanced storage stability at 4 and 22 °C.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Al-Azhar University
  • Beni-Suef University
  • Cairo University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi
  • Mikrobiologi
Originalspråkengelska
Sidor (från-till)1600-1608
Antal sidor9
TidskriftBiomacromolecules
Volym18
Utgivningsnummer5
StatusPublished - 2017 maj 8
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Adel Abouhmad, 2019 okt 9, Department of Chemistry, Lund University. 88 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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