Impact of DNA polymerases and their buffer systems on quantitative real-time PCR

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Mikrobiologi inom det medicinska området
Originalspråkengelska
Sidor (från-till)408-411
TidskriftJournal of Clinical Microbiology
Volym42
Utgåva nummer1
StatusPublished - 2004
PublikationskategoriForskning
Peer review utfördJa

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