Impact of DNA polymerases and their buffer systems on quantitative real-time PCR

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Impact of DNA polymerases and their buffer systems on quantitative real-time PCR. / Wolffs, Petra; Grage, Halfdan; Hagberg, Oskar; Rådström, Peter.

I: Journal of Clinical Microbiology, Vol. 42, Nr. 1, 2004, s. 408-411.

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TY - JOUR

T1 - Impact of DNA polymerases and their buffer systems on quantitative real-time PCR

AU - Wolffs, Petra

AU - Grage, Halfdan

AU - Hagberg, Oskar

AU - Rådström, Peter

PY - 2004

Y1 - 2004

N2 - An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.

AB - An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.

U2 - 10.1128/JCM.42.1.408-411.2004

DO - 10.1128/JCM.42.1.408-411.2004

M3 - Article

VL - 42

SP - 408

EP - 411

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 1098-660X

IS - 1

ER -