Improved In vivo Assessment of Pulmonary Fibrosis in Mice using X-Ray Dark-Field Radiography.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Improved In vivo Assessment of Pulmonary Fibrosis in Mice using X-Ray Dark-Field Radiography. / Yaroshenko, Andre; Hellbach, Katharina; Yildirim, Ali Önder; Conlon, Thomas M; Fernandez, Isis Enlil; Bech, Martin; Velroyen, Astrid; Meinel, Felix G; Auweter, Sigrid; Reiser, Maximilian; Eickelberg, Oliver; Pfeiffer, Franz.

I: Scientific Reports, Vol. 5, 17492, 2015.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Yaroshenko, A, Hellbach, K, Yildirim, AÖ, Conlon, TM, Fernandez, IE, Bech, M, Velroyen, A, Meinel, FG, Auweter, S, Reiser, M, Eickelberg, O & Pfeiffer, F 2015, 'Improved In vivo Assessment of Pulmonary Fibrosis in Mice using X-Ray Dark-Field Radiography.', Scientific Reports, vol. 5, 17492. https://doi.org/10.1038/srep17492

APA

Yaroshenko, A., Hellbach, K., Yildirim, A. Ö., Conlon, T. M., Fernandez, I. E., Bech, M., ... Pfeiffer, F. (2015). Improved In vivo Assessment of Pulmonary Fibrosis in Mice using X-Ray Dark-Field Radiography. Scientific Reports, 5, [17492]. https://doi.org/10.1038/srep17492

CBE

Yaroshenko A, Hellbach K, Yildirim AÖ, Conlon TM, Fernandez IE, Bech M, Velroyen A, Meinel FG, Auweter S, Reiser M, Eickelberg O, Pfeiffer F. 2015. Improved In vivo Assessment of Pulmonary Fibrosis in Mice using X-Ray Dark-Field Radiography. Scientific Reports. 5. https://doi.org/10.1038/srep17492

MLA

Vancouver

Author

Yaroshenko, Andre ; Hellbach, Katharina ; Yildirim, Ali Önder ; Conlon, Thomas M ; Fernandez, Isis Enlil ; Bech, Martin ; Velroyen, Astrid ; Meinel, Felix G ; Auweter, Sigrid ; Reiser, Maximilian ; Eickelberg, Oliver ; Pfeiffer, Franz. / Improved In vivo Assessment of Pulmonary Fibrosis in Mice using X-Ray Dark-Field Radiography. I: Scientific Reports. 2015 ; Vol. 5.

RIS

TY - JOUR

T1 - Improved In vivo Assessment of Pulmonary Fibrosis in Mice using X-Ray Dark-Field Radiography.

AU - Yaroshenko, Andre

AU - Hellbach, Katharina

AU - Yildirim, Ali Önder

AU - Conlon, Thomas M

AU - Fernandez, Isis Enlil

AU - Bech, Martin

AU - Velroyen, Astrid

AU - Meinel, Felix G

AU - Auweter, Sigrid

AU - Reiser, Maximilian

AU - Eickelberg, Oliver

AU - Pfeiffer, Franz

PY - 2015

Y1 - 2015

N2 - Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with a median life expectancy of 4-5 years after initial diagnosis. Early diagnosis and accurate monitoring of IPF are limited by a lack of sensitive imaging techniques that are able to visualize early fibrotic changes at the epithelial-mesenchymal interface. Here, we report a new x-ray imaging approach that directly visualizes the air-tissue interfaces in mice in vivo. This imaging method is based on the detection of small-angle x-ray scattering that occurs at the air-tissue interfaces in the lung. Small-angle scattering is detected with a Talbot-Lau interferometer, which provides the so-called x-ray dark-field signal. Using this imaging modality, we demonstrate-for the first time-the quantification of early pathogenic changes and their correlation with histological changes, as assessed by stereological morphometry. The presented radiography method is significantly more sensitive in detecting morphological changes compared with conventional x-ray imaging, and exhibits a significantly lower radiation dose than conventional x-ray CT. As a result of the improved imaging sensitivity, this new imaging modality could be used in future to reduce the number of animals required for pulmonary research studies.

AB - Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with a median life expectancy of 4-5 years after initial diagnosis. Early diagnosis and accurate monitoring of IPF are limited by a lack of sensitive imaging techniques that are able to visualize early fibrotic changes at the epithelial-mesenchymal interface. Here, we report a new x-ray imaging approach that directly visualizes the air-tissue interfaces in mice in vivo. This imaging method is based on the detection of small-angle x-ray scattering that occurs at the air-tissue interfaces in the lung. Small-angle scattering is detected with a Talbot-Lau interferometer, which provides the so-called x-ray dark-field signal. Using this imaging modality, we demonstrate-for the first time-the quantification of early pathogenic changes and their correlation with histological changes, as assessed by stereological morphometry. The presented radiography method is significantly more sensitive in detecting morphological changes compared with conventional x-ray imaging, and exhibits a significantly lower radiation dose than conventional x-ray CT. As a result of the improved imaging sensitivity, this new imaging modality could be used in future to reduce the number of animals required for pulmonary research studies.

U2 - 10.1038/srep17492

DO - 10.1038/srep17492

M3 - Article

VL - 5

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 17492

ER -